目的:建立不同产地南板蓝根药材中靛玉红含量的高效液相色谱法。方法:采用Agilent ZORBAX SB-C18色谱柱(5 μm, 4.6 ×150 mm);以甲醇-0.1%磷酸水为流动相,梯度洗脱;流速为0.8 mL·min−1;柱温为35℃;检测波长为290 nm;进样量为10 μL。结果:靛玉红的进样量在0.63~12.6 μg (r = 0.9999)范围内与峰面积呈较好的线性关系;平均回收率为101.18% (RSD为2.74%);靛玉红的含量为0.040~0.151 mg·g−1。结论:建立的南板蓝根含量测定方法操作简单、具有良好的重复性和可靠性。对南板蓝根质量控制及整体性评价具有一定的指导意义。Objective: To establish an HPLC method for the content of indirubin in Baphicacanthis Cusiae Rhi-zoma et Radix from different habitats. Methods: Agilent ZORBAX SB-C18 column (5 μm, 4.6 ×150 mm) was used. Gradient elution was performed with methanol-0.1% phosphoric acid as mobile phase. The flow rate was 0.8 mL·min−1. The column temperature was 35˚C. The detection wave-length was 290 nm. The injection volume was 10 μL. Results: The sample amount of indirubin showed a good linear relationship with the peak area in the range of 0.63~12.6 μg (r = 0.9999). The average recovery rate was 101.18% (RSD 2.74%). The content of indirubin was determined in the range of 0.040~0.151 mg·g−1. Conclusion: The established method for the determination of Baphic-acanthis Cusiae Rhizoma et Radix is simple, reproducible and reliable. It has certain guiding signif-icance for quality control and integrity evaluation of Baphicacanthis Cusiae Rhizoma et Radix.
展开▼
