HSP90α/β蛋白在胃癌组织中的表达及临床意义

摘要

目的：观察热休克蛋白90α/β( heat shock protein 90α/β,HSP90α/β)在胃癌组织中的表达及临床意义,为胃癌的早期诊断与分子病理分型提供有价值的实验资料。方法采用免疫组织化学染色结合组织芯片,观察HSP90α/β蛋白质在正常胃黏膜组织、癌旁组织及胃癌组织中的表达；然后分析HSP90α/β蛋白质在胃癌组织中表达的临床意义。结果免疫组织化学染色结果显示,胃癌组织中HSP90α/β蛋白质表达的阳性率高于正常胃黏膜组织和癌旁组织(P<0.05)；癌旁组织中表达的阳性率高于正常胃黏膜组织(P<0.05)；淋巴结转移患者组织中表达的阳性率高于无淋巴结转移者(P<0.05)；Ⅲ~Ⅳ期患者组织中表达的阳性率高于Ⅰ~Ⅱ期(P<0.05)。结论 HSP90α/β蛋白质的表达与胃癌组织的发生、分化程度、淋巴结转移及TNM分期有关,即随着胃癌组织分化程度的降低、淋巴结转移的发生及TNM分期的增加而表达升高。%Objective To verify clinical significance of the expression of heat shock protein 90α/β ( HSP90α/β) protein in gastric cancer tissue and provide valuable experiment data for gastric cancer patients on early diagnosis and clini-cal pathology grouping. Methods The expression of HSP90α/β protein was observed by immunohistochemistry staining in normal gastric mucosa tissue,cancer side tissue and gastric cancer tissue. Then the clinical pathology significance of the expression of HSP90α/βprotein was analyzed. Results The immunohistostaining results showed that the expressin posi-tive rate of HSP90α/βprotein was higher in gastric cancer tissue than that in normal gastric mucosa and cancer side tissue (P<0. 05),in cancer side tissue than that in normal gastric mucosa tissue (P<0. 05),in lymphaden metastatic gastric cancer tissue than that in no lymphaden metastatic gastric cancer tissue (P<0. 05) and inⅢ~Ⅳperiod patient gastric cancer tissue than that inⅠ~Ⅱperiod (P<0. 05). Conclusion The expression of HSP90α/βprotein was related with gastric cancer effluence,gastric cancer differentaiation level,lymphaden metastasis and TNM classification of gastric cancer. As the differentaiation level of gastric cancer tissue decreased,lymphaden metastasis of gastric cancer and TNM classifica-tion increased,the expression rate of HSP90α/β protein was also heightened.