Objective To detect Geniposide content in Zhijiang oral liquid. Methods The chromatographic column was Hypersil ODS 2 column (4. 6 mm × 250 mm, 5 μm); the mobile phase consisted of methanol∶ water (25∶ 75);the flow rate was 1. 0 ml/min; the detection wavelength was at 238 nm; the column temperature was at 30 ℃;the injection volume was 10μl. Results The values of Geniposide within the range of 1. 048-52. 40μg/ml had good linear correlation with the values of peak area (r=0. 9 999), the average recovery rate was 99. 49% with 1. 60% of relative standard deviation ( RSD) . Conclusion The high performance liquid chromatography ( HPLC) method is sim-ple and accurate, and can be used for the quality control and evaluation of Geniposide in Zhijiang oral liquid.%目的:测定栀姜口服液中栀子苷的含量。方法应用高效液相色谱法( HPLC),色谱柱:Hypersil ODS 2柱(4.6 mm ×250 mm,5μm);流动相:甲醇∶水(25∶75);流速为1.0 ml/min;检测波长为238 nm;柱温为30℃;进样量为10μl。结果栀子苷在1.048~52.40μg/ml范围内与其峰面积线性关系良好(r=0.9999),平均回收率为99.49%,相对标准偏差为1.60%。结论 HPLC用于栀姜口服液中栀子苷的含量测定操作简便、准确可靠,有助于该制剂的质量控制和评价。
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