Objective To investigate the effect of transplanting bone mesenchymal stem cells ( MSCs) in repair of rats with spinal cord injuries ( SCI) . Methods Bone marrow MSCs were isolated and cultured using whole bone mar-row cells adherence method, and models of SCI were established using free falling body hitting method. A total of 24 rats survived after model establishment, and were divided into transplantation group ( n=12 ) and control group ( n=12 ) . Transplantation group was transplanted with mesenchymal stem cells in injury sites, while control group was treated with same values of physiological saline. The recovery conditions of locomotory capabilities were evaluated using combined praxiology score, and the medullary histopathological changes, injuries and repair of nerve cells and expression of SRY gene were observed. Results The combined praxiology scores of transplantation group at different time points after the treatment were higher than those of control group (P<0. 05). In control group, there were cavities in spinal cord tissues and dead nerve fibers and cells. In transplantation group, the number of cavities was decreased, but hyperplastic tissues was increased uncreased as well as nerve fibers and cells, and SRY gene expression was found. Conclusion Transplan-tation of bone marrow MSCs may improve SCI repair.%目的 探讨骨髓间充质干细胞( MSCs)移植对大鼠脊髓损伤( SCI)修复的作用. 方法 全骨髓贴壁法分离培养骨髓MSCs,采用自由落体撞击法制作大鼠SCI模型,建模大鼠存活24只,分为移植组和对照组,每组12只,移植组在损伤部位移植培养的骨髓MSCs,对照组给予等量生理盐水. 根据联合行为学评分评价两组大鼠运动能力恢复情况,观察脊髓组织病理学变化、神经细胞损伤与修复及SRY基因的表达情况. 结果 移植组治疗后不同时间联合行为学评分均高于对照组(P<0. 05). 对照组脊髓组织中出现较多空腔,神经纤维及细胞坏死;移植组脊髓组织空泡减少,增生组织增多,神经纤维及细胞增多,且有SRY基因的表达. 结论 骨髓MSCs移植可促进大鼠SCI修复.
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