舌鳞状细胞癌组织中COX-2、VEGF、MMP-9和Bcl-2表达水平及临床意义

摘要

目的 观察舌鳞状细胞癌患者癌组织中环氧化酶-2(C OX-2)、血管内皮生长因子(VEGF)、基质金属蛋白酶-9(MMP-9)和B淋巴细胞瘤-2(Bcl-2)的表达水平,并探讨其临床意义.方法 选取2014年2月-2016年2月手术切除的40例舌鳞状细胞癌标本作为观察组,另选取同期40例正常舌黏膜组织标本作为对照组.检测2组COX-2、VEGF、MMP-9和Bcl-2表达水平,分析其与舌鳞状细胞癌临床病理参数的关系以及4种蛋白表达的相关性.结果 观察组COX-2、VEGF、MMP-9和Bcl-2蛋白阳性表达率高于对照组(P＜0.01).COX-2在有淋巴结转移组织中的阳性表达率高于无淋巴结转移组织(P＜0.05);VEGF、MMP-9与Bcl-2在有淋巴结转移与临床分期Ⅲ+Ⅳ期组织中的阳性表达率高于无淋巴结转移与临床分期Ⅰ+Ⅱ期的组织(P＜0.05).COX-2、VEGF、MMP-9、Bcl-2表达水平均呈正相关(P ＜0.05,P＜0.01).结论 COX-2、VEGF、MMP-9和Bcl-2蛋白参与舌鳞状细胞癌的生长、转移与侵袭,且四者间可能存在相互协同的作用.%Objective To observe expressions of cycloxygenase-2 (COX-2),vascular endothelial growth factor (VEGF),matrix metalloproteinases-9 (MMP-9) and b-cell leukemia-lymphoma-2 (Bcl-2) in tissues of patients with squamous cell carcinoma of tongue,and to explore its clinical significance.Methods A total of 40 samples of squamous cell carcinoma of tongue by surgical resection during February 2014 and February 2016 were selected as observation group,and another 40 cases of normal tongue mucosa tissues were selected as control group.Expression levels of COX-2,VEGF,MMP-9 and Bcl-2 were detected,and the relationship between the expressions and the clinicopathological parameters of squamous cell carcinoma of the tongue and correlation of the 4 proteins were analyzed.Results Positive expression rates of COX-2,VEGF,MMP-9 and Bcl-2 in observation group were significantly higher than those in control group (P ＜ 0.01).Positive expression rate of COX-2 in lymph node metastasis tissues was significantly higher than that in tissues without lymph node metastasis (P ＜ 0.05);positive expression rates of VEGF,MMP-9 and Bcl-2 in lymph node metastasis and clinical stage Ⅲ + Ⅳ tissues were significantly higher than those in non-lymph node metastasis and clinical stage Ⅰ + Ⅱ tissues (P ＜0.05).COX-2,VEGF,MMP-9 and Bcl-2 expressions were positively correlated (P ＜ 0.05,P ＜ 0.01).Conclusion COX-2,VEGF,MMP-9 and Bcl-2 proteins are involved in growth,metastasis and invasion of squamous cell carcinoma of tongue,and there may be a synergistic interaction between each other.