HCV胶体金法与ELISA法在输血前检测丙型肝炎抗体中的效果比较

摘要

目的：HCV胶体金法与ELISA法用于输血前检查丙型肝炎抗体检测的应用效果比较。方法：分别采用ELISA法和胶体金法对我院576名受血者的血清样本进行HCV-抗体检测，对两种检测方法的检测效果及检测用时及成本预算进行综合比较。结果：ELISA法阳性检测率高于胶体金法，但比较差异无统计学意义（P>0.05）；经胶体金法和ELISA检测为阳性的血清样本再使用RT-PCR 荧光定量法检测，胶体金法的检测符合率达到100%，显著高于ELISA法（P<0.05）；胶体金检测法所需检测时间短且检测所需成本更低。结论：与ELISA法比较，胶体金法用于检测丙型肝炎病毒的假阳性率更低，操作更方便，成本花费低，值得临床推广应用。%Objective:HCV colloidal gold method and ELISA method is used to check the application effect of hepatitis c antibody testing before blood transfusion. Methods:the ELISA method and colloidal gold method a total of 576 recipients serum samples of HCV antibody detection, two test methods for the detection effect of the comprehensive comparison and testing time and cost budget.Results:the method of ELISA positive detection rate is higher than that of colloidal gold method, but there was no statistical significance (P>0.05) difference comparison;The colloidal gold method and ELISA test for positive serum samples using the fluorescent quantitative rt-pcr method to detect, colloidal gold method detection coincidence rate reached 100%, significantly higher than that of ELISA method (P < 0.05); Colloidal gold test required for testing time is short and the cost is lower. Conclusions:compared with ELISA method, colloidal gold method is used to detect lower false positive rate of hepatitis c virus (HCV), operation more convenient, cost is low, worthy of clinical popularization and application.