巨噬细胞移动抑制因子及其受体CD74在宫颈癌中的临床意义

摘要

objective: To explore the clinical significance of the expression of macrophage migration inhibitory factor and its receptor CD74 and HPV gene in cervical cancer, finding the best laboratory screening method for cervical cancer. Methods: Thirty cases of cervical cancer were observed as experimental group, with 30 cases of patients with non- cervical lesions as control group. PCR+RDB method was used for HPV gene analysis, semi quantitative reverse transcription polymerase chain reaction (RT- PCR) was applied to assay MIF and CD74 mRNA and protein, SABC immunohistochemistry method was used to detect MIF and CD74 protein. Results: The mRNA and protein expression of MIF and CD74 in patients with cervical cancer were significantly higher than those without ( <0.05) . Also, HPV positive ratio in cervical cancer patients were significantly higher than those without ( <0.05) . The difference between the control and experimental groups of MIF and CD74 mRNA and protein expression were obviously higher than HPV gene ( <0.05) . Conclusion: Detections of MIF and CD74 mRNA and protein expression have important clinical significance in cervical cancer diagnosis.%目的：探讨巨噬细胞移动抑制因子(MIF)及其受体CD74、HPV基因分析在宫颈癌中的临床意义，寻找一种宫颈癌的最佳实验室筛查方法。方法：以30例无宫颈病变者作为对照组，30例宫颈癌患者作为实验组，借助PCR+RDB方法进行HPV基因分析、半定量反转录-聚合酶链反应（RT- PCR）法检测MIF及CD74 mRNA、免疫组织化学SABC法检测MIF及CD74的蛋白表达的表达。对比分析对照组与实验组之间HPV、MIF和CD74 mRNA及蛋白的差异。结果：宫颈癌患者组内MIF与CD74的蛋白表达、MIF与CD74 mRNA、HPV阳性基因的表达水平均显著高于无宫颈癌患者（＜0.05）；MIF与CD74的蛋白表达及mRNA的各自对照组与实验组两组间比较，差异性显著比HPV基因大（＜0.05）。结论：检测MIF及CD74 mRNA及蛋白表达在宫颈癌的诊断中有重要的临床意义。