莪术油通过诱导细胞凋亡抑制人乳腺癌 MDA-MB-231细胞增殖

摘要

Objective To study the anti-proliferative effect of Ezhu oil in human breast cancer line MDA-MB-231 cells. Methods The Ezhu oil was obtained by steam distillation. MDA-MB-231 cells were treated with different concentrations of Ezhu oil. The effects of Ezhu oil on MDA-MB-231 cells proliferation, intracellular LDH release were determined by MTT and LDH assay respectively. The apoptosis was monitored with Hoechst 33342 staining and the protein expression of Bcl-2 and Bas was determined with Western Blotting assay. Results Ezhu oil could significantly inhibite MDA-MB-231 cell proliferation and increase intracellular LDH releasing to culture medium. Hoechst 33342 staining howed that Ezhu oil induce apoptosis, decrease Bcl-2 protein expression and increase Bax protein expression. Conclusions Ezhu oil inhibits MDA-MB-231 cell proliferation by inducing apoptosis mediated by regulating Bcl-2 and Bax protein expression.%目的 探讨莪术油对乳腺癌细胞株MDA-MB-231增殖的抑制作用.方法 水蒸汽蒸馏法提取莪术油.不同浓度的莪术油处理MDA-MB-231细胞,MTT检测干预后细胞生长抑制率,LDH试剂盒检测LDH释放,Hoechst33342荧光染色测定细胞凋亡,Western Blotting测定抗凋亡蛋白Bcl-2、促凋亡蛋白Bax的表达.结果 莪术油可剂量依赖性的抑制MDA-MB-231细胞的增殖;增加细胞内LDH在细胞培养基的释放.Hoechst 33342染色显示莪术油诱导细胞凋亡,Western B1otting显示莪术油处理降低Bcl-2蛋白表达,增加Bax蛋白表达.结论 莪术油通过调节Bc1-2、Bax蛋白的表达诱导细胞株凋亡抑制乳腺癌MDA-MB-231细胞株的增殖.