手工制备浓缩血小板影响因素探讨

摘要

Objective To analyze the influence factors of handmade concentrated blood platelets and to improve the quality and the clinical injection effect of handmade concentrated platelets. Methods The blood donors not taken aspirin before donating blood in 72 hours were randomly enrolled in this study. The peripheral blood platelets counts were normal; blood were collected smoothly; then platelets concentrate were reserved by same centrifuging method (platelet-rich plasma method). The influence of centrifugal conditions and whole blood specifications on collecting platelets were analyzed. Results The 400ml whole blood under 2000rp, 2100rpm, 2200rpm, centrifugalize 5min platelets (unit: 109/L) 84. 0±14、90. 5±10. 5, 3. 89±0. 48, and 6min platelets (unit: 10°/L 86. 3±11. 5, 121. 5±17. 7, 85.0±16. 5. There-suits under 2100rpm and centrifugalized 6min have significant difference, compared with other groups; There is no obvious difference between other groups (P>0. 5). The difference between the platelets (1. 46±0. 28, 3. 53±0. 66, 4. 86± 0.71) prepared with 400, 300, 200ml whole blood respectively was not obvious, however the difference is significant compared 200ml with 400, 30 0ml (P<0. 01). Conclusion Under the same separation method, but different centrifuga-tion conditions, type of blood, the platelet concentrate prepared with 400 ml of whole blood under the condition that the rotating speed is 2100 rpm, the centrifuging time is 6 min, and the centrifuging temperature is 22 degrees centigrade has the highest content and the best collecting effect.%目的 探讨手工制备浓缩血小板的影响因素,提高手工浓缩血小板的质量和临床输注效果.方法 随机选择献血者且献血前72小时内未服用过阿斯匹林类药物,外周血小板计数正常,采血顺畅,采用相同的分离方法(富含血小板血浆法)手工制备浓缩血小板,观察不同规格的全血,或不同的离心条件,对收集血小板的影响.结果 400ml全血分别在转速22000 rpm、2100 rpm、2200 rpm离心5 min,血小板(单位:109/L)分别为(84.0±14.0)、(90.5±10.5)、(97.3±12.0)；离心6 min,血小板分别为(86.3±11.5)、(121.5±17.7)、(85.0±16.5),经统计学处理2100 rpm离心6min结果与其它组比较差异显著(P＜0.01)；其它各组相互比较无显著差异(P＞0.5).全血量分别为400、300和200ml时分离出的血小板为(1.46±0.28)、(3.53±0.66)、(4.86±0.71)；400、300ml间比较差异不显著(P＞0.05)；200ml与400、300ml间比较差异显著(P＜0.01).结论 采用相同的分离法,不同的离心条件、血液规格、对收集血小板均有影响.400毫升的全血,转速为2100rpm,6min,22℃离心制备的浓缩血小板含量最高,收集效果最好.