转移抑制基因KAI1/CD82在胃癌的表达及临床意义

摘要

目的 探讨肿瘤转移抑制基因KAI1/CD82 mRNA在胃癌组织中的转移表达及其临床意义.方法 用逆转录聚合酶链反应(RT-PCR)技术检测62例胃癌患者胃癌组织、癌旁组织及远处淋巴结组织中KAI1/CD82 mRNA的表达水平,通过测定所得KAI1/CD82基因表达率的中位数来界定,分为高表达组(31例)和低表达组(31例),比较2组临床病理特征.结果 KAI1/CD82 mRNA表达水平在TNMⅠ-Ⅱ、无肿瘤周围静脉侵袭以及无远处淋巴结转移的患者明显高于TNMⅢ-Ⅳ、有肿瘤周围静脉侵袭和有远处淋巴结转移者(P＜0.05).结论 KAI1/CD82 mRNA表达水平可能与胃癌的分化程度、浸润深度及淋巴结远处转移有关,检测KAI1/CD82 mRNA表达水平可作为预测胃癌预后的指标之一.%Objective To study the expression and significance of KAI1/CD82 mRNA in human gastric carcinoma. Methods The expression level of KAI1/CD82 mRNA was detected by reverse transcription-polymerase chain reac-tion(RT-PCR) in 62 cases' gastric carcinoma tissues, adjacent gastric tissues and beyond lymph nodes,To define 62 cases to high expression group and low expression group according to the expression level of KAI1/CD82 mRNA, and compare Clinical pathology characteristic of two groups. Results The expression level of KAI1/CD82 mRNA in cases who is TNM I - II ,have no peripheral tumor invasion veinsis and no beyond lymph nodes metastatic is higher than that in cases who is TNMIfl-IV ,have peripheral tumor invasion veinsis and beyond lymph nodes metastatic(P<0. 05) . Conclusion The expression of KAI1/CD82 mRNA may correlate to human gastric carcinoma differentiation degree, infiltration depth and beyond lymph nodes metastatic. E To detect the expression level of KAI1/CD&2 mRNA could be an index for metastasis prognosis of patients with gastric carcinoma.