乌司他丁对重症急性胰腺炎大鼠IL-8及TNF-α的影响

摘要

Objective To study the effects of ulinastatin on the expressions of IL-8 and TNF-α in rats with severe acute pancreatitis.Methods Ninety healthy male SD rats were divided into three groups:blank control group (30 rats),SAP group (30 rats) and ulinastatin treatment group (30 rats).Each group was divided into three subgroups:6h group,12h group and 24h group.SAP model was established by injecting 5％ sodiumtauro cholate via retrograde bile and pancreaticduct.The treatment groups were injected with ulinastatin via hepatic portal vein.The blank control group and SAP group was injected with physiological saline.Pathological changes were performed with the aid of hematoxylin-eosin staining.Detect the changes of serum amylase,IL-8 and TNF-α in different groups.Results Compared to the SAP group,the rats in ulinastatin treatment group had a lower pathological damage,the level of serum amylase,IL-8 and TNF-αwere significantly decreased in ulinastatin treatment groups (P＜0.05).Conclusion Ulinastatin can ameliorate the pathological damage in rats of SAP.The effect of ulinastatin on SAP may be related to decreasing the expressions of IL-8 and TNF-α.%目的 探讨乌司他丁对重症急性胰腺炎(SAP)大鼠IL-8及TNF-α表达的影响.方法 90只清洁级健康雄性SD大鼠随机分为3组:正常对照组、SAP模型组、乌司他丁治疗组,每组各30只.各组分为第6h、12h和24h三个亚组,每个亚组10只.利用5％牛磺胆酸钠逆行注射建立SAP模型.治疗组大鼠给予门静脉注射乌司他丁,正常对照组及模型组注射等量生理盐水.采用HE染色方法观察病理改变,并检测血清淀粉酶、IL-8及TNF-α的水平.结果 乌司他丁治疗组与SAP模型组比较,胰腺病理学改变明显减轻,血清淀粉酶、IL-8及TNF-α的表达水平明显降低(P＜0.05).结论 乌司他丁可以减轻SAP大鼠胰腺的病理改变,其作用可能与下调IL-8及TNF-α的表达有关.