烯脂酰辅酶A水合酶短链1对胆管癌细胞Warburg效应的影响

摘要

目的 研究烯脂酰辅酶A水合酶短链1(Enoyl-coenzyme A hydratase short chain 1,ECHS1)对胆管癌细胞Warburg效应的影响以及对人胆管癌裸鼠移植瘤生长的影响.方法 本研究分为实验组及对照组,实验组:干扰ECHS1基因;对照组:ECHS1未干扰;采用酶标仪测定ECHS1对胆管癌QBC939糖酵解代谢的影响;采用免疫组化法检测糖酵解代谢关键酶PKM2表达水平变化,分析ECHS1对胆管癌Warburg效应的影响;同时将构建的含siECHS1-siRNA的质粒转染人胆管癌细胞QBC939,建立裸鼠皮下移植瘤模型.观察siRNA干扰ECHS1表达对胆管癌细胞QBC939裸鼠移植瘤生长的影响.结果 酶标仪测定显示,实验组胆管癌细胞QBC939对葡萄糖的吸收较对照组明显减少,实验组胆管癌细胞QBC939乳酸生成降低,差异均有统计学意义(P＜0.05);免疫组化法检测结果表明,较对照组而言,干扰ECHS1可降低实验组胆管癌QBC939细胞中PKM2的表达,差异有统计学意义(P＜0.05).抑制ECHS1表达可减小实验组裸鼠皮下肿瘤大小(P＜0.05).结论 ECHS1通过调控PKM2蛋白表达影响胆管癌QBC939细胞Warburg效应,同时抑制胆管癌的增殖.%Objective To investigate the role of ECHS1 on the Warburg effect in cholangiocarcinoma and the growth of the human cholangiocarcinoma in transplanted nude mice.Methods The subjects were divided into the experi mental group and control group.ECHS1 gene was silenced in Experimental group.ECHS1 gene was normal in Control group.The effect of ECHS1 on the glucose metabolism of cholangiocarcinoma QBC939 cells was determined by using a microplate reader.The expression of PKM2 was detected in both experimental group and control group by western blot.Furthermore,we transfected ECHS1 siRNA to human cholangiocarcinoma cell line QBC939,established its subcutaneous transplantation tumor model in a nude mice and observed the effects of ECHS1 siRNA on growth of QBC939 cell in vivo.Results Microplate reader shows glucos uptaken was significantly lower than that in the control group (P＜0.05) (P＜ 0.05).Immunohistochemical results shown that interference ECHS1 can decrease PKM2 expression compared with cholangiocarcinoma QBC939 ceils in the control group (P＜0.05).Inhibit ECHS1 expression could decrease the nude mice subcutaneous tumor size (P＜0.05).Conclusion ECHS1 affects Warburg effect of cholangiocarcinoma QBC939 cells by regulating the expression of PKM2 protein and plays an important role in the growth of choriocarcinoma.inhibition of its expression by siRNA can reduce the growth of QBC939 cells in vivo.