屋尘螨抗原Der p2重组耻垢分枝杆菌的构建及其鉴定

摘要

Objective To construct and identify the recombinant mycobacterium smegmatis{ rM. S )which can secrete and express the exogenous antigen, namely dermatophagoides pteronyssinus antigen Der p2. Methods The identified PCW - Der p2 plasmid was transformed into M. S by the electroporation technology. And the expression of target gene in rM. S was identified by PCR technology. Results Through the identification by PCR,PCW - Der p2 plasmid was successfully transformed into M. S. Conclusion Der p2 - rM. S,which can express foreign protein through the secretory way,is constructed successfully.%目的 构建并鉴定能够分泌表达外源蛋白--屋尘螨抗原(Der p2)的基因重组耻垢分枝杆菌(rM.s).方法 采用电穿技术,将鉴定好的PCW - Der p2质粒转化到耻垢分枝杆菌(M.s),通过PCR鉴定目标基因在rM.s中的表达.结果 经PCR鉴定,成功将PCW - Der p2质粒转化到M.s.结论 成功构建了以分泌方式表达外源蛋白的Der p2 - rM.s.