HPLC法同时测定生脉散配方颗粒中人参皂苷Rg1和Re的含量

摘要

目的 探讨高效液相色谱法(HPLC)同时测定生脉散配方颗粒中人参皂苷Rg1和人参皂苷Re含量的可行性.方法 运用D101大孔吸附树脂,对生脉散配方颗粒水溶液进行分离、纯化,制备供试品溶液.HPLC条件为:Diamonsil TM C18色谱柱(5 μm,250 mm×4.6 mm),流动相为乙腈-0.05％磷酸溶液(20∶80),流速为1ml/min,检测波长203 nm,进样量10μl.结果 人参皂苷Rg1在0.252～2.52 μg范围内线性关系良好(r=0.9998),平均加样回收率为100.45％(RSD=1.01％);人参皂苷Re在0.249～2.49μg范围内线性关系良好(r=0.9995),平均加样回收率为98.77％(RSD=1.22％).结论 HPLC法同时测定生脉散配方颗粒中人参皂苷Rg1和Re的方法简便、准确、重复性好,可用于生脉散配方颗粒的质量控制.%Objective To explore the feasibility of high performance liquid chromatography (HPLC) in the simultaneous determination of ginsenoside Rg1 and ginsenoside Re in Shengmaisan Formula Granule.Methods D101 macroreticular resin was used to separate and purify Shengrnaisan Formula Granule solution to prepare test solution.HPLC conditions were:Diamonsil TM C18 chromatographic column(5 μm,250 mm× 4.6 mm),mobile phase acetonitrile-0.05％ phosphoric acid solution(20∶80),flow velocity one ml/min,detection wavelength 203 nm and sample size 10 μl.Results The linear relation of ginsenoside Rg1 was good within the range of 0.252～2.52 μg (r=0.9998);the average recovery rate was 100.45％ (RSD=1.01％).The linear relation of ginsenoside Re was good within the range of 0.249～2.49 μg (r=0.9995);the average recovery rate was 98.77％ (RSD=1.22％).Conclusion The simultaneous determination of ginsenoside Rg1 and Re in Shengmaisan Formula Granule by HPLC is simple and highly accurate and repeatable and may be used in the quality control of Shengmaisan Formula Granule.