Objective:To investigate the changes of oxidative damage and inflammatory reaction at different time in rats after subjected exhaustive exercise in plateau and sub-plateau environment.Methods:A total of 72 rats were randomly divided into high altitude group (n =36) and sub-high altitude group (n =36).Then each group of high altitude and sub-high altitude were randomly divided into six groups (n =6) according to sampling time.The rats were carried out weight-bearing swimming test till exhaustion.The swimming time was recorded,and MDA,T-SOD and TNF-α were determined.Results:The swimming time of high altitude group was significantly shorter than that of sub-high altitude group.The content of serum MDA and TNF-α had no obvious changes in sub-high altitude group.Compared with the control group at sub-high altitude,the content of serum T-SOD were significantly deceased at 12 h (P＜0.01) and then increased to control level at 24 h.In the high altitude,the content of serum MDA significantly increased at 6 h(P＜0.01) and was still on the rise at 12 h and 24 h,as time continues to 48 h,there was a decline but still higher than the control group (P＜0.05).The content of T-SOD was significantly deceased at 6 h (P＜0.01),when the time continues to 12 h and 24 h,it was increased but still lower than the control group(P＜0.05),then returned to control level at 48 h.The content of TNF-α was significantly inceased at 24 h (P＜0.01) and remained high at 48 h (P ＜ 0.01).Conclusion:Compared with the sub-high altitude group,rats in the high altitude group have a reduction of physical ability.There were much more oxidative damage and inflammatory reaction after exhaustive exercise.%目的:探讨大鼠在亚高原及高原环境下,经过力竭运动后不同时间内氧化损伤及炎症反应的变化.方法:72只大鼠随机分为亚高原组和高原组,每组36只,按照取样时间不同再分别将亚高原组和高原组随机分为6个亚组:相同海拔高度对照组,力竭后0、6、12、24、48 h组,每组6只.通过使大鼠进行负重游泳至力竭,记录游泳时间,检测力竭后不同时间血清中丙二醛(MDA)、超氧化物歧化酶(T-SOD)以及肿瘤坏死因子-α(TNF-α)的含量.结果:高原组力竭游泳时间明显短于亚高原组.在亚高原环境下,大鼠力竭运动后血清中MDA及TNF-α无显著变化;与亚高原对照组相比,T-SOD含量在力竭后12 h显著下降(P＜0.01),24 h时又恢复到对照组水平.在高原环境下,MDA含量在力竭运动后6h显著升高(P＜0.01),12 h、24 h时持续升高(P＜0.01),48 h时有所下降,但仍高于高原对照组(P＜0.05);T-SOD含量在力竭后6h时显著降低(P＜0.01),12h、24 h时有所升高,但仍低于高原对照组(P＜0.05),48 h时达到对照组水平;血清中TNF-α的含量在24 h时显著升高,48 h时仍维持在较高水平(P＜0.01).结论:大鼠在亚高原环境下,力竭运动后产生的氧化损伤及炎症反应较小,而在高原环境下,大鼠体能下降,经过力竭运动后,机体的氧化损伤以及炎症反应大幅度增强.
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