高脂膳食对雄性肥胖大鼠肾周脂肪水通道蛋白7表达的影响

摘要

Objective: To observe the expression of aquaporin 7 (AQP7) mRNA in perirenal adipose of dietary in-duced obese rats and explore the role of AQP7 in obesity development. Methods: 48 male Sprague-Dawley rats were ran-domly divided into the control group (C group) and the model group (DIO group), which were fed with normal and high-fat diet, respectively. Rats in DIO group were screened out according to body weights after 8 weeks and fed with high-fat diet for another 12 weeks. The rats of both groups were sacrificed after 8 and 20 weeks. Lee’s index, liver/body weight, fat con-tent and serum lipid were measured. AQP7 mRNA expression in perirenal adipose was determined by Real-time PCR. Results: At 8th week, body weight, Lee’s index, liver/body weight, fat content and serum TG levels in DIO group were higher than in C group (P<0.01 or P<0.05) , while these indicators were further increased(P<0.01) at 20th week and serum TC and LDL-C levels were also higher than in C group(P<0.01 or P<0.05). AQP7 mRNA expression in perirenal adipose of DIO group were up-regulated(P<0.01) at 8th week and down-regulated (P<0.05) at 20th week. Conclusions:AQP7 expres-sion in perirenal adipose might play different roles in obesity of different degrees.%目的：观察高脂膳食诱导肥胖模型大鼠肾周脂肪水通道蛋白7mRNA的表达情况，探讨其在肥胖发生中的作用。方法：将48只雄性SD大鼠随机分为对照组和造模组，分别喂基础饲料和高脂饲料。8周末造模组按体重筛选出高脂膳食诱导肥胖模型大鼠（DIO组）继续高脂喂养至20周。于第8周和第20周分别处死一批大鼠，计算Lee’s指数、肝/体比和脂/体比，检测血脂水平，实时定量聚合酶链反应测定肾周脂肪水通道蛋白7 mRNA表达水平。结果：8周末，DIO组大鼠体重、Lee’s指数、肝/体比、脂/体比和血清TG含量明显高于对照组（P<0.01或P<0.05），喂养至20周，上述指标均进一步明显增高（P<0.01），血清TC和LDL-C水平也升高（P<0.01或P<0.05）。DIO组肾周脂肪组织水通道蛋白7 mRNA表达水平于8周明显上调（P<0.01），20周下调（P<0.05）。结论：肾周脂肪组织水通道蛋白7可能在不同程度肥胖中发挥不同作用。