Objective To examine pl30 protein and mRNA expression in uterine papillary serous carcinoma (UPSC) and their clinical and pathologic significance. Methods A total of 10 UPSC patients (Stage I) were included, with 10 cases of high-level endometrial carcinoma of the same stage taken as the control group and 10 cases of normal proliferative stage endometrium (EM) taken as the disease control group. The level of p130 protein expression was determined by hematoxylin and eosin staining, microscopic observation, and immunohistochemistry, whereas the p130 mRNA levels were examined through real-time quantitative reverse transcriptase polymerase chain reaction. The clinicopathologic analysis was carried out in combination with clinical data. Results The pl30 protein and pl30 mRNA . Expression levels in the UPSC group (0. 46+0. 01 and 0. 56+0. 06, respectively) were apparently less than that of the normal proliferative stage endometrium group (0.91 ±0.04 and 2. 81 ±0.40, respectively; P<0. 01) and also less than those in high-level endometrial carcinoma (P<0. 05). Clinicopathologic analysis shows that all patients are post-menopausal women with symptoms of irregular vaginal bleeding and the average tumor size was 7.5cm (range: 1. 2-14. 8cm). The pathologic features are same as that of high-level ovarian papillary serous carcinoma.Conclusion Reduced pl30 protein and pl30 mRNA expression in UPSC might correlate with poor prognosis in UPSC patients.%目的 探讨p130蛋白和mRNA在子宫乳头状浆液性癌(UPSC)中的表达及其临床病理意义.方法 收集10例Ⅰ期UPSC患者标本作为UPSC组,10例相同分期高级别子宫内膜癌作为病例对照组,另取相同年龄10例正常增生期子宫内膜标本作为正常对照组.采用HE染色、光镜观察和免疫组织化学方法检测p130蛋白表达水平,实时定量RT-PCR方法检测p130mRNA表达水平,结合临床随访资料进行临床病理分析.结果 UPSC组p130蛋白和mRNA表达(分别为0.46±0.01、0.56±0.06)均明显低于正常增生期子宫内膜(分别为0.91±0.04、2.81±0.40,P＜0.01)和高级别子宫内膜癌(分别为0.55±0.03、0.64±0.03,P＜0.05).临床病理分析显示,患者均为绝经后女性,症状均为绝经后阴道不规则出血,肿瘤中位大小7.5cm(1.2～14.8cm),病理学特征与卵巢高级别乳头状浆液性癌相似.结论 UPSC中p130蛋白和mRNA表达减少,可能与UPSC患者预后较差有关.
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