Objective To investigate the effects and mechanism of triptolide (TPL) on proliferation and apoptosis of human cerical cancer CaSki cells in vitro. Methods CaSki cells were cultured with 0.5, 10 and 20ng/ml TPL for 24.48 and 72 hours, the cell proliferation was then observed by MTT assay, the morphological characteristics were observed by inverted phase contrast microscopy, and the expression of Ki-67 and p53 protein was determined by immunohistochemistry. Results Inverted phase contrast microscopy showed the CaSki cells in TPL treated groups were shrinkage and round, with reduced volume and rough surface, cell number declined, intercellular space dilated, and the changes were aggravated with the time elapsed and the TPL concentration increased. The 5,lO and 20ng/ml TPL showed inhibitory effects on the proliferation of CaSki cells in vitro in a time- and dose-dependent manner. Immunocytochemical staining showed that the expression of Ki-67 and p53 protein decreased in a time- and dose-dependent manner in CaSki cells cultured with TPL (P＜O.Ol). Conclusion TPL may effectively inhibit the proliferation and induce apoptosis of CaSki cells, and the mechanism may be related to the down-regulation of Ki-67 and p53 protein expression.%目的 探讨雷公藤内酯醇(TPL)对人宫颈癌CaSki细胞增殖和凋亡的影响及其作用机制.方法 采用MTT比色法检测不同浓度(0、5、10、20ng/ml)TPL作用24、48、72h后对CaSki细胞增殖的影响,倒置相差显微镜观察细胞形态学变化,细胞免疫化学法检测Ki-67及p53蛋白表达的改变.结果 倒置相差显微镜脱察可见TPL处理后细胞皱缩变圆变粗糙,体积缩小,且不断地脱落悬浮于培养液中,数量减少,细胞间隙增大,且随药物浓度增加及作用时间延长,这种变化趋于明显.5、10、20ng/ml TPL在体外对CaSki细胞的生长具有抑制作用,且呈剂量、时间依赖方式(P＜0.01).免疫细胞化学检测显示TPL作用后Caski细胞Ki-67及p53蛋白表达下调,且呈剂量、时间依赖方式(P＜0.01).结论 TPL可抑制CaSki细胞增殖并诱导其凋亡,其机制可能与抑制癌细胞Ki-67及p53蛋白表达有关.
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