高效液相色谱串联质谱法测定人血浆中西酞普兰含量

摘要

Objective To establish an HPLC - MS/MS method for delerminalion of the conlenl of cilalopram in human plasma. Methods The chromalographic column was Agilenl - ZORBAX - C8 (4. 6 × 150 mm,5 μm) , from Agilenl Company, The mobile phase was melhyl cyanides -0.l%formic acid(60:40). The flow rale was 0. 5 ml/min and m/z 256.2→467. 1. The column tem-peralure was 20 ℃. ElecLrospray ionizalion( ESI) source was applied and multi - reaclion moniloring( MRM) mode wilh the lransilions of m/z 325. 1→262. L(for cilalopram) and m/z 256.2→467. 1 (for diphenhydramine inlernal slandard)was used. Results The assay linearily of cilalopram was confirmed over the range 0. 5 - 100 ng/ml ( r = 0. 9979) . The inlra - and inler - day precision ( RSD) was less lhan 10%. Conclusions The melhod is proved lo be quick,specific,sensilive and accurate,which can be used for the delerminalion of cilalopram in plasma.%目的 探讨应用高效液相色谱串联质谱法(HPLC-MS/MS)测定血浆中西酞普兰药物浓度的方法.方法 设立色谱柱为Agilent-ZORBAX-C8柱;流动相:乙腈-0.1%甲酸水溶液(60:40);流速:0.5 ml/min;柱温:20 ℃;质谱条件为电喷雾电离源(ESI),以多反应监测(MRM)方式进行检测,用于定量分析的反应离子为m/z 325.1 262.1(西酞普兰)和m/z 256.2 67.1(苯海拉明,内标).结果 西酞普兰在0.5~100 ng/ml范围内线性关系良好(r=0.9979);西酞普兰的日内、日间精密度RSD均＜10%.结论 该方法分析速度快,专属性强,灵敏度高,可作为西酞普兰的血浆药物浓度检测方法.