促溃疡愈合细胞/水凝胶三维复合物的初步构建及表征

摘要

目的：应用壳聚糖衍生物水凝胶负载表达人血小板衍化生长因子-BB 的 CHO 细胞系，初步构建促溃疡愈合复合物。方法：选择壳聚糖经 N-乙酰-L-半胱氨酸修饰后合成含巯基的双硫键壳聚糖，加水合成水凝胶，用 MTT 法检测生物相容性并负载转基因 CHO 细胞，构建促溃疡愈合复合物并进行表征。结果：该水凝胶材料与转基因 CHO 细胞系生物相容性良好。水凝胶呈网格结构，孔径一致、细胞黏附生长，负载转基因 CHO 细胞构成三维复合物。结论：CHO-rhPDGF-BB 细胞具有新的生物学功能，水凝胶与该细胞相容性良好，为研究糖尿病溃疡创面愈合提供了实验基础。%　　Objective :Use natural macromolecule hydrogel material to compose 3D cell scaffolds loading transgenic cell that could stably express platelet-derived growth factor-BB (PDGF-BB) to construct accelerating ulcer concrescence compound.Method:Choosed chitosan and modified it by NAC to synthesize disulfide bond chitosan with sulfydryl,dissolved the compound into deionized water generating hydrogel,tested it’s cytotoxicity by MTT;used it loading transgenic CHO as 3D cell scaffolds to construct cell/scaffold composition,scaned it’s structure.Result:The hydrogel has good biocompatibility.The compound has network structure,aperture well-distributed,cells stuck well.Conclusion:The CHO cell line had new biological character,and successfully constructed the cell/scaffold composition,which can afford experiment base and theory foundation to study ulcer concrescence.