雷尼酸锶联合强骨胶囊对大鼠骨质疏松性骨折骨愈合影响的实验研究

摘要

Objective:To explore the significance of treatment of combining traditional Chinese and western medicine on osteoporotic fracture,and to observe the effect of Strontium ranelate combined with Qianggu capsule on osteoporotic fracture healing. Method:30 spragne-dawley rats were given with the retinoie acid intragastrically to induce osteoporosis.All rats with osteoporosis were randomly divided into the experimental group and the control group,each group had 15 rats,the experimental group and the control group were respectively given Strontium ranelate combined with Qianggu capsule and normal saline. The rats in each group were sacrificed at the second,fourth and sixth week respectively. Size of bony callus was measured and histopathologic features were analyzed.Result:There was no significant difference of the size of bony callus between two groups at the second week(P>0.05). At the fourth week,the experimental group’s bony callus was smaller than the control group and there was significant difference between two groups (P<0.05). At the sixth week,the experimental group’s bony callus was obviously smaller than the control group and there was significant difference between two groups(P<0.01). At the second week,histological inspection found that compared with the control group,there was no significant increased of osteoblast number in the experimental group,but at the fourth week and the sixth week,there was significant increased. Conclusion:Strontium ranelate combined with Qianggu capsule has obviously curative effect on osteoporotic fracture rats caused by retinoie acid.%目的：通过观察雷尼酸锶联合强骨胶囊对骨质疏松性骨折愈合的影响，以探讨中西医结合治疗骨质疏松性骨折的意义。方法：30只SD大鼠给予维甲酸灌胃，制造骨质疏松性骨折模型成功后，按随机数字表分为实验组和对照组，每组15只。实验组给予雷尼酸锶联合强骨胶囊灌胃，对照组给予生理盐水灌胃，于2周、4周和6周处死实验鼠，进行骨痂大小的测量及组织学研究。结果：2周时两组骨痂大小比较差异无统计学意义（P>0.05）；4周时实验组骨痂大小较对照组小，差异有统计学意义（P<0.05）；6周时实验组骨痂大小明显小于对照组，差异有统计学意义（P<0.01）。2周时组织学检测发现，实验组成骨细胞数目较对照组无明显增多；4周及6周时则明显增多。结论：雷尼酸锶联合强骨胶囊治疗维甲酸所致大鼠骨质疏松性骨折疗效显著。