重组抗人表皮生长因子受体（EGFR）嵌合单克隆抗体CH225非还原电泳纯度分析

摘要

目的：分析CH225在非还原电泳时抗体条带主条带以外在高分子量和低分子量产生杂条带的原因。方法：在不同的供试品缓冲液和不同电泳条件下进行SDS-PAGE电泳比较。结果：上样前100℃煮样1 min与加碘乙酰胺结果相同。结论：高分子量和低分子量杂条带可以用电泳上样前100℃煮样1 min消除即可。%Objective:To analyze the non-Reduced SDS-PAGE purity of monoclonal antibody CH225 and the reason of the appearance of bands with a lower or a higher molecular weight.Method:The EGFR samples were treated with various loading buffers and various conditions for SDS-PAGE.Result:The result of incubation of 100℃for 1 min appeared the same to the result with iodoacetamide added.Conclusion:The appearance of the bands with a lower or a higher molecular weight may be eliminated by incubation of 100℃for 1 min for SDS-PAGE.