维生素D受体基因多态性与慢性乙型肝炎患者的关联研究

摘要

Objective:To investigate the association of vitamin D receptor gene Fok Ⅰ polymorphisms with the clinical phenotype of chronic hepatitis B patients. Method:The genotype of vitamin D receptor gene Fok Ⅰ of 44 heavy degree chronic hepatitis B patients,46 moderate degree chronic hepatitis B patients and 40 non-active HBsAg carriers were tested by polymerase chain react ion-restriction fragment length polymorphism(PCR-RFLP). Result:There were significant differences in FokI genotypes among heavy degree,moderate degree chronic hepatitis B patients and non-active HBsAg carriers(P<0.01);There were significant difference in the frequence of allel F/f among heavy degree,moderate degree chronic hepatitis B patients and non-active HBsAg carriers(P<0.01),Pearson C=0.53 and the frequency of alleles f in the two groups was significantly higher than that in non-active HBsAg carriers.Conclusion:Vitamin D receptor gene polymorphisms have an relationship with the clinical phenotype of chronic hepatitis B patients.%目的：探讨维生素D受体（VDR）FokⅠ基因多态性与慢性乙型肝炎患者临床表型的关联。方法：应用聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）技术检测VDR FokⅠ基因的多态性在44例慢性乙型肝炎重度患者、46例中度患者和40例非活动性HBsAg携带者中的分布，并进行基因型分析。结果：慢性乙型肝炎重度组、慢性乙型肝炎中度组VDR FokⅠ基因的基因型与对照组（非活动性HBsAg携带者）比较差异有统计学意义（P<0.01）；慢性乙型肝炎重度组、中度组与对照组VDR FokⅠ基因酶切位点的等位基因F/f分布存差异有统计学意义（P<0.01）。Pearson列联系数=0.53，提示等位基因F/f分布与慢性乙型肝炎患者的不同临床表型存在相关性。慢性乙型肝炎重度组、中度组f等位基因分布频率高于对照组。结论：VDR FokⅠ基因的多态性与慢性乙型肝炎患者的不同临床表型存在一定的关系。