缺氧缺血性脑病新生儿外周血淋巴细胞钟基因表达的变化

摘要

Objective:To study the effect of circadian rhythm disorder induced by HIE in clock genes through the comparison changes of hypoxic ischemic encephalopathy full-term neonates and normal neonates in the peripheral blood lymphocytes cell (PBLC) in biological clock gene Clock mRNA, Bmal1 mRNA levels.Method:32 patients of HIE and 24 normal full-term newborns were determinated by semi quantitative reverse transcription polymerase chain reaction (RT-PCR) in PBLC Clock mRNA, Bmal1 mRNA,the difference between groups were compared.Result:In groups HIE the levels of Clock mRNA and Bmal1 mRNA displayed significantly lower than the control groups, the difference was statistically significant(P<0.05),the changes trend of Bmal1 mRNA and Clock mRNA in peripheral blood lymphocytes of HIE groups were basically in the same.Conclusion:The expression of Clock mRNA and Bmal1 mRNA were abnormal in PBLC of HIE group, which may plays a certain role in hypoxia and ischemia occurred after the disorder of circadian rhythm.%目的：通过比较足月缺氧缺血性脑病患儿与正常足月新生儿外周静脉血中淋巴细胞（Peripheral blood lymphocytes cell，PBLC）中钟基因Clock mRNA、Bmal1 mRNA含量水平的变化，研究探讨HIE所致的昼夜节律紊乱中对钟基因的影响。方法：采用半定量逆转录聚合酶链反应（Reverse Transcriptase Polymerase Chain Reaction，RT-PCR）测定32例缺氧缺血性脑病患儿（HIE组）与24例正常足月新生儿（对照组）外周血淋巴细胞中Clock mRNA、Bmal1 mRNA的含量，并比较各组之间的差异。结果：HIE组外周血淋巴细胞中Clock mRNA和Bmal1 mRNA 的水平均低于对照组，两组比较差异有统计学意义（P<0.05），HIE组外周血淋巴细胞中Clock mRNA、Bmal1 mRNA含量的变化趋势基本一致。结论：HIE患儿PBLC中Clock mRNA、Bmal1 mRNA表达存在异常，两者可能在缺氧缺血后昼夜节律紊乱的发生起着一定的作用。