一种新的大鼠脊髓半离断损伤模型

摘要

Objective:To investigate a lateral spinal cord injury model with less trauma and better replication. The model would be usefull for the study of various new medicines or stem cell therapy on spinal cord injury.Method: A modified self-made needle was used to insert into lumbar spinal cord of SD rats without opening the cavum medullare,the lateral spinal cord injury was established.The grasping ability (movement function) and sensory function by temprature of hind-limbs were measured and compared with contralateral side at different time points after injury for insure the model was successfully.Result:It showed that lateral spinal cord injury model of rats appeared obvious lateral paralysis ,but without incontinence of urine and defecation. Also none of them had infection locally or systemically. The model was successfully.Conclusion:The spinal injure model made by self-made modified needle insertion into SD rats lumbar spinal cord is easy to handle ,repeat,and induce obvious lateral paralysis without severe complications.Due to its advantage of connecting, some medicines or stem cells could be injected conveniently at the same time while surgery processed,then provides an experimental basis to explore the molecular mechanism of spinal cord injury regeneration difficulty.%目的：探讨一种对机体影响较小、复制性良好的大鼠脊髓损伤模型，为后期药物及干细胞干预脊髓损伤后修复提供条件。方法：采用改良注射针头连接注射器作为损伤工具，在不打开脊髓腔的前提下沿脊椎棘突左侧垂直插入脊髓，造成大鼠腰髓一侧离断性损伤。除观察动物的一半行为学改变外，并对脊髓损伤后对大鼠的感觉功能（热痛反应）、运动功能（抓力测试）变化进行定时观测，以确定模型复制成功。结果：大鼠麻醉苏醒后即刻出现一侧后肢瘫痪、拖地行走，但无排尿及排便失禁，动物一般状态良好，后期未见合并感染，表明动物模型复制成功。结论：用自制的注射器针头造成的脊髓损伤模型操作简便、损伤较小、重复好，并且因与其折射相连，可在造模同时局部给予药物或试剂进行干预，为探索脊髓损伤再生困难的分子机制提供了实验基础。