Objective To establish a method for preparing hvdroxvsaf Ior yel ow A (HSYA) from Carthamus tinctorius L. Methods Aqueous extract prepared through vacuum evaporation was added to D-101 macroporous resin column ,then HSYA was eluted by water and 10% ethanol.High performance liquid chromatography(HPLC) was used to determine the content of HSYA. Results 3.2883g HSYA was obtained from 700g crude drug with the purity of 96.86%. Conclusion The method is simple and feasible and can be used to prepara HSYA from Carthamus tinctorius L.%目的建立红花中羟基红花黄色素A(hydroxysaf lor yel ow A,HSYA)的制备方法。方法红花水提液浓缩后上D101型大孔树脂,在水和10%乙醇部分得到HSYA,采用高效液相色谱法(HPLC)进行了含量测定。结果从700g红花药材中分离得到6.5687gHSYA,纯度达到96.86%。结论此方法操作简单,可作为HSYA的制备工艺。
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