微纳米生物活性玻璃的细胞基因激活性能研究

摘要

Microano biological material has become the research hotspot and difficulty in the field of biomedical mate-rials.A number of studies have shown that microano structure of biological material can show a positive biological re-sponse.Bioactive glass ( BG) has the high apatite-forming bioactivity and biocompatibility.BG is the ideal biomaterial for bone tissue regeneration.Microano-bioactive glass ( MNBG) caused much attention because of the special morphology and physicochemical properties, but the research about MNBG only focused on preparation and the in vitro apatite-forming bioactivity, few papers have focused on the biocompatibility and gene activation .This study used sol-gel method combining biological organic templates to design microano structure of bioactive glasses.The effects of MNBG and traditional melt 45S5 ionic dissolution products on proliferation, expression of osteogenic genes, proteins were investigated.The results showed that MNBG promoted cell-proliferation, up-regulated the expression of osteogenic genes and proteins.This work will provide a reference for design of specific morphology of the bioactive bone repair materials in the future .%微纳米生物材料目前已成为生物医用材料领域一个研究热点和难点。大量研究表明具有微纳米结构特征的生物材料表现出了积极的生物学响应。生物活性玻璃（ BG）具有较高的生物活性、生物相容性，是一类重要的骨修复材料。而微纳米生物活性玻璃（ MNBG）因其具有特殊的形态结构和理化性能，引起众多研究者的关注。但是目前对 MNBG的研究还主要集中在制备、表征以及其表面类骨羟基磷灰石矿物在SBF溶液中的形成活性等方面，关于MNBG的细胞相容性以及基因激活性能方面的研究还鲜有报道。通过溶胶-凝胶法结合模板仿生技术合成了具有特殊微纳米结构和形态的MNBG，并将其浸提液与MG-63细胞共培养，研究生物玻璃溶出物对细胞增殖，成骨相关基因和蛋白表达的影响，结果证明相比于传统的熔融法制备的生物玻璃（45S5）浸提液， MNBG浸提液能够明显促进细胞增殖，激活细胞成骨相关基因，上调相关蛋白的表达，为设计和制备具有基因介导作用的新型生物活性玻璃骨修复材料提供了理论依据。