桃花水母的核糖体RNA基因ITS区序列分析及物种鉴定

摘要

The ribosomal RNA geneinternal transcribed spacer (ITS) region ofCraspedacustafrom Qingdao was isolated using the polymerase chain reaction followed by sequencing. Homology and phylogenetic analyses re-vealed that theITS region ofCraspedacustashowed a high similarity withCraspedacusta sowerbyi(>93%). Si-multaneously, our results showed that this region is closely related toC. sowerbyi using a phylogenetic tree. The present data suggest that theCraspedacustafrom Qingdao are closely related toC. sowerbyi. This is a new study aboutCraspedacusta in Qingdao.%利用 PCR 扩增和 DNA 测序技术，获得了青岛野外采集到的桃花水母(Craspedacusta)核糖体RNA 基因 ITS 区序列。序列比对以及系统进化分析显示，在青岛采集到的桃花水母与索氏桃花水母(Craspedacusta sowerbyi)的ITS区序列相似度最高，同源性在93%以上；同时，在系统发育树中也与索氏桃花水母聚为一支。研究结果表明，青岛采集到的桃花水母为索氏桃花水母，这也是青岛地区桃花水母的新记录。