耐酸酒精酵母的诱变选育研究

摘要

In this experiment, acidproof alcohol yeast strain A3 was used as original strain and its protoplast was treated by UltraViolet(UV)-Nitrosoguanidine (NTG)composite mutation to obtain acid-proof and high ethanol-producing mutants. then a quality strain UN1-81 was finally screened by three-class screening mode from large quantity of flat plates. The mutated strain after fermentation could obtain 11.17%(Vol) alcohol concentration which enhanced 16.47 % to start strain, and the stable genotype with high ethanol yield had been verified through twenty times of transferring of culture.%以耐酸性酒精酵母A3为出发菌株，对其原生质体进行紫外线（UV）与亚硝基胍（NTG）复合诱变，利用三级筛选模式，筛选出一株高产突变菌株UN1-81。该菌株在发酵结束后，酒精度达到11.17%vol，比出发菌株提高了16.47%以上，且经过20次传代培养，酒精产量稳定。