用FISH技术研究人类体外未受精卵的21号染色体非整倍体

摘要

采用荧光原位杂交技术,选用人类21号染色体端粒探针(21qter), 检测人类体外未受精卵的21号染色体非整倍体发生率, 并比较非整倍体率与25～30岁和31～35岁这两个女性年龄组、IVF指征、超排方案之间的关系 .在54个未受精卵中,正常21号单体30枚,二体16枚,三体4枚,缺体4枚,非整倍体率为44 .4%(24/54);25～30岁和31～35岁这两个年龄组、IVF指征、超排方案的患者的21号染色体非整倍体率之间的差异无显著性.卵母细胞21号染色体的非整倍性是造成体外受精失败的重要原因之一.%Telomeric probe of 21qter was us ed by st andard FISH procedures to study chromosome 21 aneuploidy in the human oocytes fa iling to fertilize in vitro ,to analyze the relationship between the differe nt women′s age,IVF indications,ovarian stimulation protocols and the frequency of chromosome 21 aneuploidy . Of 54 human unfertilized oocytes, 30 were haploid (55.6%),16 were disomic(29.6%), 4 were trisomic (7.4%), 4 were nosomic (7.4%).The overall frequency of aneuploidy was 44.4%.The chromosome 21 aneuploidy was one of the factors to cause in vitro fertilization failure of human oocytes .