Many biological products were manufactur ed by means of large-up culture of animal cells. To increase the cell-specific productivity and to facilitate purification, serum-free media supplemented wit h several nutriments were used for cell culture, and microcarriers were chosen in favor of cell growth and high cell density. Bioreactors with simple manipula tion, good qualification and aeration were adopted. More suitable conditions fo r cell growth could be given, and higher expression level could be gained thro ugh on-line supervising the environment for cell growth and decreasing restrain t factors in cell culturing. The expression of anti-apoptosis genes using recom binant DNA technology could increase cell viability and productivity. The beads -to-beads transfer method of porous microcarriers was emphasized in large-up culture of animal cells.%利用动物细胞大规模培养技术可生产多种生物制品.为提高细胞活力和表达水平及有利于表达产物的纯化,采用有多种添加成分的无血清培养基培养细胞,选择更有利于细胞生长又可提高培养细胞密度的微载体和条件温和、易操作、气体交换速度快的生物反应器.在线监控细胞生存环境和生理活动,减少培养过程培养基中的抑制因素,可创造更适合细胞生存的环境,提高表达水平.向细胞中导入抗凋亡基因,可提高细胞活性和蛋白产量.利用多孔微载体以球转球方式大规模培养动物细胞有很好的发展前景.
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