胃癌中CDC4和cyclinE的表达及临床意义

摘要

To investigate the expression of CDC4 and cyclin E in gastric cancer and elucidate the potential relationship between their expression and clinical pathological characteristics.Semiquantitative reverse transcription polymerase chain reaction (RT-PCR) was determined the mRNA expression of CDC4 and cyclin E in part of random selecting gastric cancer samples and corresponding adjacent normal mucosal tissues; Im-munohistochemistry was used to detect the protein expression of CDC4 and cyclin E in 60 specimens of gastric cancers and corresponding adjacent normal mucosal tissues.The expression of CDC4,cyclin E and the clinical pathological characteristics were analyzed.The protein expression of CDC4 in gastric cancer tissues were significantly lower than that in adjacent normal mucosal tissues (P<0.05),whereas the protein expressionof cyclin E in gastric cancer tissues were significantly higher than that in adjacent normal mucosal tissues (P<0.01).The protein and mRNA expression of CDC4,cyclin E were correlated with differentiation,TNM stage,lymph node metastasis,infiltration.There was a significant negative correlation between CDC4 and cyclin E at the mRNA expression levels,cyclin E overexpression is likely to be the CDC4IFBXW1 loss.The loss of CDC4 may be a valuable marker for assessing the diagnosis and treatment and the prognosis of gastric canzcer.%检测胃癌中CDC4/Fbxw7、cyclinE的表达,分析其与胃癌临床病理特征的关系和临床意义,采用逆转录多聚酶链反应(RT-PCR)检测部分胃癌和胃正常组织中CDC4、cyclinE mRNA的表达；免疫组化(SP法)检测60例胃癌组织及对应正常组织中CDC4/FBXW7、cyclin E蛋白的表达,探讨两者的临床病理特征之间的关系.胃癌中CDC4蛋白表达显著低于正常组织(P＜0.05),而cyclin E蛋白的表达在胃癌组织中显著增高,阳性率与癌旁正常组织比较,差异有统计学意义(P＜0.01）.CDC4、cyclin E蛋白和mRNA表达均与胃癌的分化程度、TNM分期、淋巴结转移及浸润深度有关.CDC4、cyclinE在mRNA表达水平上呈负相关.CDC4的表达缺失可能导致cyclin E的过表达.CDC4的低表达可能是胃癌诊疗及预后判断的重要生物学指标.