Ligation of bilateral common carotid arteries in rats is usually used to create chronic cerebral ischemia model.But the advantages of making this model by using FeCl3 remain unclear.Here,four model groups of rats were treated with FeCl3 at contents of 20％,35％,50％ and 70％,respectively,and the control group was treated with the ligation method.Mortality rate of rats,neurological function score,HE staining,Y-maze test and the weight of the common carotid artery thrombosis were used to evaluate the model's stability and success rate.The results demonstrated that the death rates of model groups treated with 20％,35％ and 50％ concentrations were 5％,15％,10％,respectively,significantly lower than that of the ligation group (x2=8.533,P=0.003;x2=4.286,P=0.038;x2=6.144,P=0.013).Compared with the sham group,the model groups and ligation group had no significant change (P＞0.05) in neurological function score.HE staining results showed that the concentrations of 35％ and 50％ can form a better model of arterial stenosis.Y-maze training of the model groups (35％,50％) had no significant change compared with the ligation group (P＞0.05).The weights of thrombus from low concentration group were significantly decreased compared with that from the middle/high concentration group (P＜0.01),but there was no significant change between 35％ and 50％ model groups (P＞0.05).These data suggested that this method can improve the success rate and stability of chronic cerebral ischemia model,and that the best choice of concentration is 35％ or 50％.%慢性脑缺血模型通常采用结扎大鼠双侧颈总动脉的方法,但采用FeCl3法制作模型的优势尚无研究.实验以不同浓度FeCl3 (20％,35％,50％,70％)处理颈总动脉的方法作为模型组,以结扎大鼠颈总动脉的方法作为对照组,制备大鼠慢性脑缺血模型,并通过动物死亡率、神经功能评分、颈总动脉HE染色、Y迷宫实验以及颈总动脉血栓的质量验证模型的稳定性和成功率.研究发现低、中浓度FeC13组死亡率(5％,15％,10％)与结扎组(45％)相比显著降低(x2=8.533,P=0.003;x2=4.286,P=0.038;x2=6.144,P=0.013).结扎组和FeC13组神经功能评分与假手术组相比无显著差异(P＞0.05).不同浓度FeCl3对颈总动脉病理学影响的HE染色结果显示,中浓度能形成较好的动脉狭窄模型.中浓度Ⅰ组和中浓度Ⅱ组Y迷宫正确反映次数与结扎组相比无显著差异(P＞0.05).低浓度组血栓质量较中浓度和高浓度组显著降低(P＜0.01),中浓度Ⅱ组较中浓度Ⅰ组无显著差异(P＞0.05).结果表明本方法与结扎法效果相同且能提高模型成功率,同时制备的模型可靠,脑缺血状态稳定,并以中浓度(35％,50％)为宜.
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