目的:了解北京市通州区副溶血性弧菌的耐药情况,用以指导临床治疗用药及防控措施的制定.方法:选取通州区2009～2011年在食品监测和门诊病人中分离收集的60株副溶血性弧菌,采用微量内汤稀释法,测定菌株对23种抗生素的最小抑菌浓度,并用碱裂解法和脉冲场凝胶电泳检测菌株的质粒谱,用PCR方法检测菌株中整合子及SXT元件的携带情况.结果:菌株对阿莫西林、氨苄西林、盘尼西林和多粘菌素等4种抗生素的敏感性低于40％,对妥布霉素有5％的中度耐药,对其余18种抗生素100％敏感；质粒携带率低,仅2株菌SXT整合酶阳性,Ⅰ、Ⅳ类整合子全部阴性.结论:北京市通州区副溶血性弧菌的耐药不严重,除2类抗生素外,其余抗生素在副溶血性弧菌感染的治疗中都有效.%Objective: Antibiotics resistance was investigated in the Vibrio parahaemolyticus isolates from Tongzhou district of Beijing to provide helpful guide for clinical treatment and prevention of food poisoning of V.parahaemolyticus. Methods: A total of 60 V.parahaemolyticus strains, isolated from Tongzhou district between 2009 and 2011 were selected and their minimal inhibitory concentrations of 23 clinical commonly used antibiotics were tested by the micro-dilution assay. The present of plasmid and integrom that may be relative with drag resistance were also analyzed. Results: The sensitivities of four antibiotics were lower than 40%, which were amoxicillin, am-picillin, polymyxin and penicillin. For tobramycin, there were 5% intermediate strains. All of strains were sensitive to other 18 antibiotics. A few of plasmids were presented in the strains including two plasmids bigger than 30 kb, and two strains were SXT element positive. Conclusion: The antibiotics resistance of V.parahaemolyticus isolates in Tongzhou district was not serious. Except ampicillin and polymyxin, others were effective for treatment of infections of V.parahaemolpicus.
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