SD大鼠缓解-复发型实验性变态反应性脑脊髓炎模型的建立

摘要

Objective: To investigate establishing the relapsing-remitting model and pathological characteristics of experimental allergic encephalomyelitis(EAE) model, and to provide the experimental value for researching multi⁃ple sclerosis(MS). Methods: The animal model were established in SD rats by immunization with complete Freund adjuvant(CFA) and GPSCH and boosted once. The clinical manifestation was observed. Inflammatory cell infiltration and demyelination were detected by HE stain and fast blue stain respectively. Results: Over 80% rats developed to EAE in group treated with GPSCH and CFA but none of the control groups. The inflammation cell were found around vessels and demyelination in white matter of cerebellum and spinal cords. Conclusion: Relaps⁃ing-remitting EAE can be induced in SD rats by immunization with the homogenate of GPSCH and CFA.%　　目的：建立大鼠缓解-复发型实验性变态反应性脑脊髓炎(EAE)模型,进行病理学研究,为多发性硬化(MS)的研究提供据.方法：采用大鼠脊髓和弗氏完全佐剂混合乳剂一次性注入 SD 大鼠双足垫和尾部,1周后半剂量注射进行一次加强,诱导大鼠发生 EAE；观察发病情况,HE 染色观察病理变化,监牢兰染色观察脱髓鞘情况.结果：空白对照组大鼠均未出现症状,HE 染色小脑及脊髓无炎性细胞浸润,监牢兰染色未见髓鞘脱失；模型组临床症状发生率为90%以上,HE 染色可见小脑白质及脊髓血管周围有大量炎性细胞浸润,监牢兰染色发现有大片髓鞘脱落.结论：用 SD 大鼠脊髓髓鞘蛋白和弗氏完全佐剂混合乳剂可诱导同种 SD 大鼠发生缓解-复发型 EAE.