溶瘤腺病毒CD55-TRA IL-IETD-MnSOD联合阿霉素抑制肝癌细胞HepG2生长

摘要

Objective:To study the effect of oncolytic adenovirus CD55-TRAIL-IETD-MnSOD combined with doxorubicin hydrochloride(DOX) on the growth of hepatocellular carcinoma cells HepG2.Methods:MTT method was used to detect HepG2 survival after treatment CD55-TRAIL-IETD-MnSOD combined with DOX.Cell morphological changes were detected by Hoechst33342 staining and flow cytometry technology to detect the number of apoptotic cells.Western blotting was used to detect apoptotic pathways triggered by combined treatment.Results:MTT experiment showed that through combination of CD55-TRAIL-IETD-MnSOD and DOX can inhibit the growth of HepG2 cells in a synergic way and more effectively.Hochest dyeing experiments and flow cytometry technology results showed CD55-TRAIL-IETD-MnSOD and DOX enhance the induction of cell apoptosis.Western blot verifled CD55-TRAIL-IETD-MnSOD combined with DOX activated Caspase apoptosis pathway.Conclusion:DOX can promote the replication of CD55-TRAIL-IETD-MnSOD,therefore,the apoptosis pathway of Caspase can be effectively activated,and the HepG2 growth can be inhibited.%目的:研究溶瘤腺病毒CD55-TRAIL-IETD-MnSOD与阿霉素联合使用对肝癌细胞HepG2生长的影响.方法:MTT法检测经CD55-TRAIL-IETD-MnSOD与阿霉素联合处理后HepG2细胞生存率的变化,Hoechst33342染色及流式细胞术检测联合处理诱导细胞凋亡时细胞形态变化及凋亡细胞数量,Western印迹检测联合处理所引发的凋亡通路.结果:MTT实验显示,联合使用CD55-TRAIL-IETD-MnSOD和阿霉素能更有效地抑制HepG2细胞的生长,且阿霉素与CD55-TRAIL-IETD-MnSOD是协同作用;Hochest染色实验和流式细胞术实验结果均显示联合使用CD55-TRAIL-IETD-MnSOD和阿霉素增强了诱导细胞凋亡的作用;Western印迹表明CD55-TRAIL-IETD-MnSOD和阿霉素联合使用激活了Caspase细胞凋亡途径.结论:阿霉素能促进溶瘤腺病毒CD55-TRAIL-IETD-MnSOD的复制,联合阿霉素和溶瘤腺病毒CD55-TRAIL-IETD-MnSOD可更有效激活Caspase细胞凋亡通路,抑制肝癌细胞HepG2生长.