连翘叶的喇曼光谱鉴别

摘要

为了通过喇曼光谱对不同采收期的连翘叶进行快速质量评价,采用RM-1000型共焦显微喇曼光谱仪获取其喇曼光谱图后,对其喇曼图谱进行比较的方法,进行了理论分析和实验验证,取得了不同采收时间的连翘叶喇曼光谱特征峰强度的数据.结果表明,连翘叶的喇曼峰主要位于700cm-1～1600cm-1和2600cm -～3 l00cm -1范围内,这些特征喇曼峰和其主要有效成分连翘苷密切相关,可以用于连翘叶的定性鉴别；不同采收时间连翘叶的喇曼特征峰强度存在明显差异,可以用于连翘叶的质量评价.喇曼光谱对连翘叶的定性鉴别和质量评价具有直接快速、简单准确的优势,这一结果对中药的质量控制是有帮助的.%In order to assess the quality of the forsythia leaves gathered in different periods rapidly, their Raman spectra were obtained with RM-1000 confocal microscopy laser Raman spectrometer, then the specific peaks of their Raman spectra were obtained after analysis and experiments. The Raman spectra of forsythia leaves show the main characteristic peaks are mainly in the range of 700cm-1 ~ 1600cm-1 and 2600cm-1 ~ 3100cm-1. The characteristic peaks are closely related with forsythin which is considered as the main active ingredient in the forsythia leaves and can be used for qualitative identification. The peak intensity in the spectra of forsythia leaves gathered in different periods is significantly different so that the quality of forsythia leaves can be evaluated. Raman spectra can be used for qualitative identification and evaluation of forsythia Leaves and it is direct, fast, simple and accurate. The results are useful for quality control of the traditional Chinese medicine.