ICP-AES分析高脂饮食兔肾脏组织的微量元素含量

摘要

目的：探讨实验性动脉粥样硬化（ AS）兔肾脏组织中铁、锰、铜、锌、硒、铅和镉元素含量的变化。方法采用高脂饮食复制兔AS模型，获取肾脏，用硝酸-过氧化氢混合液微波消解样本，用电感耦合等离子体原子发射光谱法（ ICP-AES）分析肾脏组织中铁、锰、铜、锌、硒、铅和镉的含量。结果正常对照组和高脂饮食组血清总胆固醇（ TC）和肾脏组织中锌、铁、硒、镉含量差异均有统计学意义（ P＜0．05）。两组肾脏组织镉元素含量均明显高于饲料样本（ P＜0．05）。结论高脂饮食兔肾脏锰、铜、锌、硒元素含量降低可能与食物摄取和脂蛋白代谢异常有关。镉元素容易在肾脏中蓄积。%Objective To investigate the content changes of Fe, Mn, Cu, Zn, Se, Pb and Cd in rabbit kidney with atherosclerosis ( AS) .Methods The model of rabbit kidney with AS was reproduced by a high-adipose diet, kidney was obtained, the samples were microwave-digested with miscible liquids of nitric acid and peroxide of hydrogen, and then the Fe, Mn, Cu, Zn, Se, Pb and Cd contents were determined by inductive coupled plasma atomic emission spectrometry (ICP-AES).Results Serum total cholesterol (TC) and Zn, Fe, Se and Cd contents in normal control and high-adipase groups had statistical significance (P<0.05).Cd contents of the 2 groups were significantly higher than that in feed sample (P<0.05).Conclusions Mn, Cu, Zn and Se contents in high-adipose group decline, which may have some relation with the intake of foods and the abnormality of lipoprotein metabolism.Cd accumulates easily in kidney.