3种鉴定系统在微生物室间质量评价中的应用和比较

摘要

目的 评价基质辅助激光解析电离飞行时间质谱(MALDI-TOF MS)、Vitek 2 Compact全自动微生物鉴定系统(简称Vitek 2 Compact)和MicroScan WalkAway 96 plus全自动微生物鉴定系统(简称MicroScan WalkAway 96 plus)在质量控制中的鉴定应用价值.方法 采用MALDI-TOF MS、Vitek 2 Compact和MicroScan WalkAway 96 plus同时对61株不同批次的质控菌株进行鉴定并作比较.结果MALDI-TOF MS、Vitek 2 Compact和MicroScan WalkAway 96 plus对26株革兰阴性杆菌的鉴定准确率分别为88.4%、100.0%和84.6%,对15株革兰阳性球菌的鉴定准确率分别为100.0%、100.0%和86.7%,对8株酵母样真菌的鉴定准确率分别为100.0%、100.0%和87.5%,对12株其他细菌的鉴定准确率分别为100.0%、83.3%和41.7%.结论 MALDI-TOF MS、Vitek 2 Compact和MicroScan WalkAway 96 plus 3个鉴定系统对常见的革兰阳性球菌、革兰阴性杆菌、酵母样真菌的鉴定准确性较好.在苛养菌和革兰阳性杆菌方面, MALDI-TOF MS和Vitek 2 Compact的鉴定准确性较MicroScan WalkAway 96 plus好.%Objective To evaluate matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS),Vitek 2 Compact and MicroScan WalkAway 96 plus automatic microbial identification systems in microbiological external quality assessment.Methods A total of 61 isolates of quality control strains with different runs were identified by MALDI-TOF MS,Vitek 2 Compact and MicroScan WalkAway 96 plus. The results of 3 identification systems were compared.Results The accuracy rates of MALDI-TOF MS,Vitek 2 Compact and MicroScan WalkAway 96 plus for identifying 26 isolates of Gram-negative bacilli were 88.4%,100.0% and 84.6%, the accuracy rates for identifying 15 isolates of Gram-positivecocci were 100.0%,100.0% and 86.7%,the accuracy rates for identifying 8 isolates of yeast-like fungi were 100.0%,100.0% and 87.5%,and the accuracy rates for identifying other 12 isolates were 100.0%,83.3% and 41.7%,respectively.Conclusions The accuracy rates of the 3 identification systems for Gram-positive cocci,Gram-negative bacilli and yeast-like fungi are good. For fastidious bacteria and Gram-positive bacilli,the accuracy rates of MALDI-TOF MS and Vitek 2 Compact are higher than those of MicroScan WalkAway 96 plus.