不同剂量吡格列酮对膀胱癌上皮细胞生长的影响

摘要

Objective To study the interventional effects of different doses of pioglitazone on the growth of epithelium cells in the patients with bladder cancer .Methods A total of 60 cases of bladder cancer were selected and randomly divided into the low dose group(n= 30) and the high dose group(n= 30) .1 μmol/L of pioglitazone and 10μmol/L of pioglitazone were respectively adopted to conduct the interventional therapy in the two groups .The surviv‐al rate of bladder cancer epithelium cells was detected on 1 ,2 ,3 ,4 d of interventional therapy .The expression levels ofα‐SMA mRNA and CTGF mRNA ,and collagen Ⅲ level were detected on 4 d of interventional therapy .The results were performed the statistical analysis .Results the cells survival rate of bladder cancer epithelium cells in the two groups was significantly decreased along with the interventional therapy duration extending ,and the difference was statistically significant(P< 0 .05) ,moreover the survival rates of bladder cancer epithelium cells on 2 ,3 ,4 d of inter‐ventional therapy in the high dose group were significantly lower than those in the low dose group ,the difference was statistically significant(P< 0 .05) .The expression levels of α‐SMA mRNA ,CTGF mRNA and collagen Ⅲ level in the high dose group were significantly lower than those in the low dose group ,the difference was statistically significant (P< 0 .05) .Conclusion Higher dose of pioglitazone has better effect than low dose of pioglitazone ,which may fur‐ther reduce the expression levels of α‐SMA mRNA and CTGF mRNA ,and collagen Ⅲ level ,thus induces the apopto‐sis of bladder cancer epithelial cells .%目的：研究不同剂量吡格列酮对膀胱癌上皮细胞生长的干预效果。方法选取膀胱癌患者60例，将其随机分为低剂量组（n＝30）和高剂量组（n＝30），分别采用1μmol／L 和10μmol／L 吡格列酮进行干预治疗。分别在干预治疗1、2、3、4 d 时检测膀胱癌上皮细胞存活率，在干预治疗4 d 时检测α‐平滑肌肌动蛋白（α‐SMA）mRNA和结缔组织生长因子（CTGF）mRNA 表达水平及Ⅲ型胶原浓度，对结果进行统计分析。结果随着干预治疗时间的延长，两组患者膀胱癌上皮细胞存活率均明显降低，差异有统计学意义（P＜0．05），且在干预治疗2、3、4 d 时高剂量组膀胱癌上皮细胞存活率低于低剂量组，差异均有统计学意义（P＜0．05）。高剂量组患者的α‐SMA mRNA 、CT‐GF mRNA 表达水平和Ⅲ型胶原浓度均显著低于低剂量组，差异均有统计学意义（P＜0．05）。结论较高剂量的吡格列酮可能通过进一步降低α‐SMA mRNA 、CTGF mRNA 表达水平和Ⅲ型胶原浓度，从而诱导膀胱癌上皮细胞的凋亡，效果优于低剂量吡格列酮。