胶体金法与全自动生化分析仪检测肌酸激酶同工酶的浓度比较

摘要

Objective To explore the correlation and difference between the colloidal gold method and the fully automatic biochemical analyzer for detecting the creatine kinase isoenzyme(CK‐MB) .Methods 327 serum specimens from the patients with myocardial injury in our hospital from January 2013 to December 2014 were analyzed .The col‐loidal gold method and the fully automatic biochemical analyzer were adopted to measure the CK‐MB concentration of the same sample .The corresponding relation and difference of the detection results were compared between the two methods .Results The coincidence rate of the sample number for the CK‐MB activity range of 30 - 110 U /L detected by the fully automatic biochemical analyzer and which for the CK‐MB concentration range of 5 .0 - 20 .0 ng/mL dis‐playing as “ + ” detected by the colloidal gold method was 95 .97% ;the coincidence rate of the sample number for the CK‐MB activity range of 110 - 450 U /L detected by the fully automatic biochemical analyzer and which for the CK‐MB concentration range of 20 .0 - 100 .0 ng/mL displaying “ + + ” detected by the colloidal gold method was 95 .41% ;the coincidence rate of sample number for the CK‐MB activity range over 450 U /L and which for the CK‐MB concentration range over 100 ng/mL displaying “ + + + ” detected by the colloidal gold method was 94 .68% . Conclusion The concentration range prompted by the serum CK‐MB color intensity detected by the colloidal gold method has certain proportional corresponding relation with the CK‐MB activity range detected by the fully automatic biochemical analyzer .%目的：探讨胶体金法与全自动生化分析仪检测肌酸激酶同工酶（CK‐MB）的相关性及差异，为临床应用提供依据。方法选取2013年1月至2014年12月重庆市精神卫生中心歌乐山院区收治的心肌损伤患者血清标本327例，采用胶体金法及全自动生化分析仪检测同一血清标本 CK‐MB 浓度及活性，比较2种方法测定结果的相关性及差异。结果全自动生化分析仪测出的 CK‐MB 活性范围为30～110 U ／L 的标本数量与胶体金法测定CK‐MB 显示“＋”（浓度范围5．0～20．0 ng／mL ）的标本数量符合率为95．97％；全自动生化分析仪测出的 CK‐MB活性范围为110～450 U ／L 的标本数量与胶体金法测定 CK‐MB 显示“＋＋”（浓度范围20．0～100．0 ng／mL）的标本数量符合率为95．41％；全自动生化分析仪测出的 CK‐MB 活性范围大于450 U ／L 的标本数量与胶体金法测定的CK‐MB 显示“＋＋＋”（浓度范围大于100 ng／mL）的标本数量符合率为94．68％。结论胶体金法测定的 CK‐MB的显色强度提示的浓度范围与全自动生化分析仪测定的血清 CK‐MB 活性范围呈一定比例的对应关系。