目的：探讨抗‐HCV阳性和抗‐HCV阴性与丙型肝炎核酸（HCV‐RNA）载量关系及其在丙型肝炎检测中的应用价值。方法回顾性分析2010年9月至2013年10月该院236例丙型肝炎患者，抽取所有患者血清标本，采用化学发光微粒子免疫法检测抗‐HCV ，实时荧光定量聚合酶链反应法检测 HCV‐RNA ，并对检测结果进行比较。结果抗‐HCV总检出率为83．4％（197／236），HCV‐RNA总检出率为87．1％（207／236），两者比较差异有统计学意义（P＝0．00），抗‐HCV和HCV‐RNA联合检测的准确率为98．7％（233／236）。HCR‐RNA阳性和阴性患者ALT阳性率比较，差异有统计学意义（P＝0．00）。HCR‐RNA和ALT相关系数为 r＝0．86（P＝0．00）。结论联合检测抗‐HCV和 HCV‐RNA ，可早期确诊 HCV感染患者。%Objective To explore the relationship between the positive result of Hepatitis C virus antibody (anti‐HCV) ,negative result of anti‐HCV and HCV‐RNA loads and its application in detecting patients with HCV . Methods A retrospective analysis was performed 236 patients with HCV from September 2010 to October 2013 .The anti‐HCV was detected by the method of Chemiluminescence Immunoassay .HCV‐RNA was tested by real‐time fluo‐rescencequantitative polymerase chain reaction .Results The detection rate of anti‐HCV was 83 .4% (197/236) ,and that of HCV‐RNA was 87 .1% (207/236) ,the difference in the two groups was statistical different (P=0 .00) .The accuracy rate of combined detection of Anti‐HCV and HCV‐RNA in diagnosis of HCV was 98 .7% (233/236) .The difference of alanine aminotransferase(ALT ) level between the patients with positive result of HCV‐RNA and pa‐tients with negative result of HCV‐RNA was statistical significant (P=0 .00) ,HCR‐RNA and ALT were positively correlated (r=0 .86 ,P<0 .05) .Conclusion The combined detection of anti‐HCV and HCV‐RNA is value for early diagnosis for HCV .
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