首页> 中文期刊> 《检验医学与临床》 >脂肪血制备悬浮红细胞的质量研究

脂肪血制备悬浮红细胞的质量研究

         

摘要

目的:研究脂肪血制备的悬浮红细胞的质量变化。方法按常规制备滤除白细胞悬浮红细胞的方法处理轻、中、重度脂肪血及正常血,检测其过滤前后红细胞渗透脆性,观察红细胞在储存期 K +、Na +浓度、pH 值、游离血红蛋白浓度的变化。结果 中、重度组红细胞渗透脆性在过滤后明显增高,轻度组、正常组无明显变化。储存期中、重度组红细胞 K +浓度、游离血红蛋白浓度明显高于轻度组和正常组(P<0.05)。 Na+浓度、pH 值各组间比较差异无统计学意义(P>0.05)。 结论对于轻度脂肪血可采用弃其血浆,利用红细胞,而对于中、重度脂肪血应该选择报废。%Objective To explore the quality change of suspended leukocyte‐reduced red blood cells (SL‐rRBCs) prepared from lipid blood and to provide experimental evidence to develop reasonable and effective methods to reduce the scrappage of lipid blood .Methods SLrRBCs were prepared from light ,moderate and severe lipid blood by conventional method ,and RBC osmotic fragility was detected before and after filtering .Then the changes of the concentrations of K + ,Na+ and free Hb and the pH values of different SLrRBCs were analyzed during storage period . Results RBC osmotic fragility of moderate and severe groups were increased ,but no change was observed of light and normal groups .The concentrations of K + and free Hb of moderate and sever groups were higher than those of light and normal groups(P< 0 .05) ,and there were no difference of the concentration of Na + and the pH value during storage period(P> 0 .05) .Conclusion RBC of light lipid blood could be utilized ,but moderate and severe lipid blood should be scrapped .

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