乙二胺四乙酸依赖性假性血小板减少症简易纠正方案

摘要

目的：乙二胺四乙酸（EDTA）依赖性假性血小板减少症标本通过肝素锂、枸橼酸钠及改良方案进行纠正，对血小板测定结果进行评估，建立一个简单、实用、且不延长结果报告时间（TAT）的方法。方法征集ED‐TA依赖性假性血小板减少症志愿者120例，同时收集肝素锂、枸橼酸钠、EDTA‐K3抗凝标本各一份，其中EDTA‐K3标本采用改良方法处理后再检测，三组检测结果与参考方法的测定结果进行比较。评估差异有无统计学意义。结果肝素锂、枸橼酸钠、改良方案的纠正率分别为80．5％、39．2％、100．0％；与参考方法比较，P值分别0．129、0．013、0．051。T A T分别为（2．03±0．29）、（1．8±0．35）、（0．47±0．19）h。结论肝素锂组的结果与参考方法测定结果相关性最好，可比性最好。其次是改良组和枸橼酸钠组。改良方案对 TAT影响最小、不用进行患者的二次采血，并且结果与枸橼酸钠相似，因此该改良方案可以用于临床上对PTCP标本的纠正。%Objective To correct the specimens of EDTA‐dependent pseudothrombocytopenia(PTCT ) by a‐dopting heparin lithium ,sodium sitrate and the improved corrective solution and to evaluate the platelet detection re‐sults for establishing a simple ,practical method without extending the results report time .Methods 120 volunteer of EDTA‐dependent PTCT and each anti‐coagulation specimen with EDTA‐K3 ,heparin lithium and sodium citrate were collected ,in which the specimens of EDTA‐K3 was treated by the improved method and then the detection was con‐ducted .The detection results in 3 groups were compared with the detection results by the reference method and whether having the statistical significance was evaluated .Results The corrective rates of heparin lithium ,sodium cit‐rate and improved corrective solution were 80 .5% ,39 .2% and 100 .0% .Their P values were 0 .129 ,0 .013 and 0 .051 respectively .Their result report time(TAT) were(2 .03 ± 0 .29) ,(1 .8 ± 0 .35) ,(0 .47 ± 0 .19) h respectively .Conclu‐sion The results of the heparin lithium group has the best correlation and the detection results by the reference method and the best comparability ,followed by the improved group and the sodium citrate group .The improved cor‐rective scheme has the minimal influence on TAT and without needing to collect blood specimen twice ,its detection results are close to those detected by the sodium citrate method .Therefore the improved corrective scheme can be used to correct the PTCP specimens in clinic .