目的 探讨洗涤人血小板在体外对革兰阳性球菌代表菌(金黄色葡萄球菌)及革兰阴性杆菌代表菌(大肠埃希菌)活性的影响.方法 分离和洗涤人血小板,加入20倍的细菌共孵育3 h.将共孵育后的反应液梯度稀释后涂平板,计数细菌菌落形成单位(CFU)来判断活菌数量;将共孵育后反应液离心,检测上清液中Th1/Th2/Th17细胞因子,分析与细菌作用后的血小板细胞因子释放情况.结果 金黄色葡萄球菌和大肠埃希菌与血小板共孵育后,细菌CFU数目均明显下降.血小板对金黄色葡萄球菌和大肠埃希菌的杀菌率分别为96.8％ 和40.5％.金黄色葡萄球菌可以促进血小板产生γ-干扰素和白细胞介素-17a,而大肠埃希菌没有显示出明显作用.结论 洗涤血小板能够明显抑制金黄色葡萄球菌和大肠埃希菌的活性,并释放出较高浓度的炎性细胞因子.%Objective To discuss the effects of washed platelets on activity of the G + bacteria (Staphylococcus aureus) and G-bacteria (Escherichia coli) .Methods Human platelets were seperated ,washed ,and incubated for 3 hours with the above bacteria of 20 times quantity .After co-incubation ,the reaction solution was diluted and plated on the agar ,and the bacterial colony forming u-nits(CFU) were counted to determine the number of living bacteria .Moreover ,Th1/Th2 /Th17 cytokines in the supernatant were detected by CBA after centrifugation of the reaction solution .Results After incubation with Staphylococcus aureus and Escherichia coli ,washed platelets presented significant decrease of bacterial CFU counts .The bactericidal rates of platelets to Staphylococcus aureus and Escherichia coli were 96 .8％ and 40 .5％ ,respectively .In addition ,Staphylococcus aureus could promote the production of IFN-γ and IL-17a released by platelets ,while Escherichia coli did not show any obvious effect .Conclusion Washed platelets can significantly inhibit the activity of Staphylococcus aureus and Escherichia coli and release high concentration of inflammatory cyto-kines .
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