茶多酚联合万古霉素对耐甲氧西林金黄色葡萄球菌生物膜的影响

摘要

Objective To explore the effects of tea polyphenols single use and its combined use with vancomycin on the biofilms of methicillin-resistant staphylococcus aureus(MRSA).Methods Ninety-six-hole polypropylene cell culture dish was adopted to de-tect the biofilm formation ability of different sources of MRSA,the antimicrobial activity of tea polyphenols against MRSA was de-tected by the agar dilution method,moreover the role of tea polyphenols single use and its combination with vancomycin on the bio-film formation was observed.Results The different sources of MRSA all formed the biofilms(average range 0.64 -1.15 in each A595group)in a certain degree,among them,the biofilm formation ability in the strains(A595was 1.15)from the wound secretion was most strong;tea polyphenols had certain antibacterial activity to MRSA strains,the minimum inhibitory concentration range was 0.08-0.64 mg/mL;vancomycin could reduce the biofilm formation of MRSA to some extent,tea polyphenols single use had weaker impact on the biofilm elimination,but the combination use of the two materials could increase the biofilm formation.Conclu-sion Tea polyphenol combined with vancomycin can increase the biofilm formation of MRSA strains,prompting that clinic should pay attention to the effect of tea polyphenols when treating the MRSA biofilm related infection.%目的 探究茶多酚单用及其与万古霉素联合作用对耐甲氧西林金黄色葡萄球菌(M RSA)生物膜的作用.方法 采用96孔聚丙烯细胞培养皿检测不同来源M RSA的生物膜形成能力,通过琼脂稀释法检测茶多酚对M RSA的抗菌活性,并观察测量茶多酚单用及其与万古霉素联合作用对M RSA生物膜的作用.结果 不同标本来源的M RSA均可在一定程度形成生物膜(各组生物膜A595为0.64~1.15),其中伤口分泌物来源的菌株生物膜形成能力最强(A595为1.15);茶多酚对M RSA有一定的抗菌活性,最低抑菌水平为0.08~0.64 mg/mL;万古霉素可以一定程度降低M RSA的生物膜形成,茶多酚单用对生物膜消除影响较弱,但是二者联合使用可以增加生物膜形成.结论 茶多酚联合万古霉素可增加M RSA菌株生物膜的形成,提示临床治疗M RSA生物膜相关感染时,需注意茶多酚物质的影响.