板蓝根多糖在结核小鼠模型中的免疫干预研究

摘要

目的 研究板蓝根多糖(IIP)在结核小鼠模型中对Th细胞的免疫干预效果.方法 选取健康SPF小鼠50只,分为三组,对照组10只,其他40只经尾静脉注射标准菌株H37RV混悬液建立结核小鼠模型,其中模型组10只,干预组30只.干预组小鼠造模成功后分别予IIP 100、200、300 mg/(kg·d)腹腔注射,观察三组小鼠的临床表现,造模成功时及成功后20、30 d后各抽取1次外周血检测干扰素-γ(IFN-γ)、白细胞介素-4(IL-4)、IL-12水平.结果 干预组小鼠症状较模型组小鼠缓解,外周血检测IFN-γ、IL-4、IL-12水平先升高后降低,差异均有统计学意义(P<0.05).结论 IIP可通过对Th细胞的干预对结核感染起到积极作用.%Objective To research the immune intervention effect of isatidis indigotica polysaccharide (IIP)on Th cells in tuber-culosis(TB)mouse model.Methods Fifty healthy SPF mice were selected and divided into 3 groups,10 cases were in the control group and other 40 cases were given standard bacterial strain H37RV mixed suspension solution by tail vein injection for construc-ting the TB mouse mode,in which 10 cases in the model group and 30 cases in the intervention group.The mice in the intervention group were intraperitoneally injected by IIP 100,200,300 mg/(kg·d).Then the clinical manifestations were observed in 3 groups. Peripheral blood was collected at the time of model construction success and on 20,30 d after model construction success.The levels of interferon-γ(IFN-γ),interleukin-4(IL-4)and IL-12 were detected by using ELISA.Results The symptoms in the intervention group were relieved compared with the model group,the levels of IFN-γ,IL-4 and IL-12 were increased first and then decreased,the difference was statistically significant(P <0.05).Conclusion IIP play a positive role to TB infection by intervening Th cells.