IL-1β、IL-6和IL-18在颅脑损伤患者中的表达及其临床意义

摘要

目的 探究白细胞介素(IL)-1β、IL-6和IL-18在颅脑损伤患者中的表达及其临床意义.方法 选取2012年12月至2014年12月在该院接受治疗的颅脑损伤患者163例(颅脑损伤组),其中轻度68例,中度49例,重度46例.分别留取损伤后0、1、4、7、15、30 d的血液标本.另选取健康人57例作为对照组,采用酶联免疫吸附试验检测损伤后各个时间点IL-1β、IL-6和IL-18的水平.结果 与对照组和治疗后比较,颅脑损伤组治疗前IL-1β、IL-6和IL-18表达水平均显著增高,差异有统计学意义(P<0.05);与对照组比较,颅脑损伤组治疗后IL-1β、IL-6和IL-18表达水平差异无统计学意义(P>0.05).颅脑损伤组患者的3种炎性因子水平自入院起逐渐增加,至治疗后4 d最高,再逐渐降低,至治疗后30 d时3种炎性因子水平已低于入院时水平,差异有统计学意义(P<0.05).在任意时间点,轻度颅脑损伤患者的3种炎性因子水平均低于中重度颅脑损伤患者,差异有统计学意义(P<0.05).结论 血清IL-1β、IL-6和IL-18表达水平会随着颅脑损伤的严重程度变化,可作为判断颅脑损伤严重程度的指标.%Objective To explore the expression of inflammatory cytokines(IL-1β,IL-6 and IL-18) in craniocerebral injury and its clinical significance.Methods A total of 163 patients with craniocerebral injury(craniocerebral injury group) in our hospital form December 2012 to December 2014 were selected,including 68 mild cases,49 moderate cases and 46 severe cases.Blood samples were collected on 0,1,4,7,15,30 d after injury.Other 57 healthy people were selected as the control group.The levels of IL-1β,IL-6 and IL-18 at each time point were detected by adopting the enzyme-linked immunosorbent assay.Results Compared with the control group and after treatment,the levels of IL-1β,IL-6 and IL-18 before treatment in the craniocerebral injury group were significantly increased,the difference was statistically significant(P<0.05);compared with the control group,the levels of IL-1β,IL-6 and IL-18 after treatment in the craniocerebral injury group had no statistical difference(P>0.05).The levels of IL-1β,IL-6 and IL-18 in the craniocerebral injury group were gradually increased from the admission,and reached to the highest on 4 d after treatment,then gradually decreased,and were lower than those at admission on 30 d,the difference was statistically significant(P<0.05).The levels of IL-1β,IL-6 and IL-18 at each time point 30 days in the mild injury patients were lower than those in the moderate injury patients,the difference was statistically significant(P<0.05).Conclusion The expression levels of serum IL-1β,IL-6 and IL-18 may be changed with the severity degree of craniocerebral injury,which may serve as the indicator for the severity of craniocerebral injury.