胰岛素样生长因子结合蛋白相关蛋白1表达对鼻咽癌细胞系C N E1发生发展的研究

摘要

Objective To investigate the inhibitory effect of insulin-like growth factor binding protein relat-ed protein 1(IGFBP-rP1)on proliferation,invasion and migration of nasopharyngeal carcinoma cell line CNE1.Methods The construction of two small interfering RNA specific for IGFBP-rP1 gene(siRNA32,siR-NA34)and negative control siRNA,CNE1 cells were divided into four groups:siRNA32 group(transfected with siRNA32 transfection),siRNA34 group(transfected with siRNA34),negative control group(negative control siRNA transfection)and blank group(no treatment).After training 24 h or 48 h,the related indexes were detected.Results The expression of IGFBP-rP1 mRNA and protein in siRNA32 group and siRNA34 group was significantly lower than that in negative control group and blank group(P<0.05).The number of CNE1 cells in siRNA32 group and siRNA34 group was higher than that in PVE-F membrane(P<0.05).The OD value of CNE1 cells in siRNA32 group and siRNA34 group was significantly lower than that in negative control group and blank group(P< 0.05).The OD value of CNE1 cells in siRNA32 group and siRNA34 group was significantly lower than that in negative control group and blank group(P<0.05).The percentage of G0/G1phase and G2/M phase cells in siRNA32 group and siRNA34 group was significantly higher than that in negative control group and blank group(P<0.05)was lower than the negative control group and the blank group(P<0.05).Conclusion Inhibition of IGFBP-rP1 expression can reduce the proliferation,invasion and migration of nasopharyngeal carcinoma CNE1 cells,and provide a new idea for the clinical treatment of naso-pharyngeal carcinoma.%目的 探讨胰岛素样生长因子结合蛋白相关蛋白1(IGFBP-rP1)对鼻咽癌细胞系CNE1细胞的增殖、侵袭、迁移能力的影响.方法 构建2条特异性针对IGFBP-rP1基因的小干扰RNA(siRNA32、siRNA34)及其阴性对照siRNA,将CNE1细胞分为4个转染组:siRNA32组(siRNA32转染)、siRNA34组(siRNA34转染)、阴性对照组(阴性对照siRNA转染)、空白组(不做任何处理),分别培养24 h或48 h后检测相关指标.结果 siRNA32组和siRNA34组IGFBP-rP1 mRNA及蛋白表达水平显著低于阴性对照组和空白组(P<0.05);siRNA32组和siRNA34组CNE1细胞迁移数、穿过PVP-F膜的CNE1细胞数显著低于阴性对照组和空白组(P<0.05);培养24、48 h后,siRNA32组和siRNA34组CNE1细胞增殖OD值显著低于阴性对照组和空白组(P<0.05);培养48 h后,siRNA32组和siRNA34组G0/G1期、G2/M期细胞所占比例显著高于阴性对照组和空白组(P<0.05);siRN A32组和siRN A34组S期细胞所占比例显著低于阴性对照组和空白组(P<0.05).结论 抑制IGFBP-rP1表达能降低鼻咽癌CNE1细胞的增殖、侵袭、迁移能力,为临床治疗鼻咽癌提供一种新的思路.