CarbAcineto NP快速检测耐碳青霉烯酶的鲍曼不动杆菌的方法学评价

摘要

Objective To evaluate the method of fast detecting carbapenemase of acinetobacter baumannii. by CarbAcineto NP.Methods A total of 80 strains were used to evaluate the performance of test from Januar-y 2015 to January 2016 in the Third Hospital of Hebei Xing Tai.Identification and susceptibility testing of 80 strains of A.baumannii was determinated by VITEK compact.CarbAcineto NP was applied to detect the Car-bapenemase of the A.baumannii and the OXA-23 gene of the Carbapenemase was detected by the common PCR method.Results There were 49 of 80 strains resistant to imipenem and meropenem.PCR showed that 47 of 49 were carrying OXA-23 gene,43 of 49 strains was positive for CarbAcineto NP experiment,31 of 80 in A. baumannii strains was susceptible to imipenem and meropenem.PCR showed that OXA-23 gene was negative in 29 of 31 and CarbAcineto NP was negative in 30 of 31 non-carbapenemase-producing A.baumannii.The sen-sitivity and the specificity of CarbAcineto NP was 87.8% and 96.7%,respectively.Conclusion CarbAcineto NP was concordance with results obtained by PCR to detect gene coding for OXA-23.The CarbAcineto NP can detect rapidly carbapenemase producing in acinetobacter baumannii%目的 快速检测产碳青霉烯酶的鲍曼不动杆菌的方法学评价.方法 选择邢台市第三医院2015年1月至2016年1月在临床标本中连续收集的80株鲍曼不动杆菌,采用全自动细菌鉴定仪对鲍曼不动杆菌进行药敏试验,应用普通PCR方法对所有菌株进行OXA-23型碳青霉烯酶基因检测,同时应用CarbAcineto NP方法检测菌株的碳青霉烯酶.结果 80株鲍曼不动杆菌中,49株耐亚胺培南和美罗培南,其中47株菌检测出OXA-23基因,43株菌CarbAcineto NP为阳性;31株菌对亚胺培南和美罗培南敏感,其中29株菌OXA-23阴性,30株菌CarbAcineto NP为阴性.CarbAcineto NP方法的敏感性为87.8%,特异性为96.7%.结论 与普通PCR方法比较,CarbAcineto NP方法的试剂成本较低,易于操作,时间短,能快速有效检测鲍曼不动杆菌的OXA-23型碳青霉烯酶.